Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human USP20 protein (Tagged)
See all USP20 proteins and peptides
Specific Activity: 10 pmol/min/μg
Assay Conditions: 50mM Tris-HCl, 0.5mM EDTA, 0.05% Tween 20, 1mM DTT, 500nM Ubiquitin-AMC. Incubate at room temperature for 30 minutes. Fluorescence intensity is measured at exc/em of 360nm/460nm using Tecan M1000 plate reader.
Purity>= 90 % SDS-PAGE.
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceGDSRDLCPHL DSIGEVTKED LLLKSKGTCQ SCGVTGPNLW ACLQVACPYV GCGESFADHS TIHAQAKKHN LTVNLTTFRL WCYACEKEVF LEQRLAAPLL GSSSKFSEQD SPPPSHPLKA VPIAVADEGE SESEDDDLKP RGLTGMKNLG NSCYMNAALQ ALSNCPPLTQ FFLECGGLVR TDKKPALCKS YQKLVSEVWH KKRPSYVVPT SLSHGIKLVN PMFRGYAQQD TQEFLRCLMD QLHEELKEPV VATVALTEAR DSDSSDTDEK REGDRSPSED EFLSCDSSSD RGEGDGQGRG GGSSQAETEL LIPDEAGRAI SEKERMKDRK FSWGQQRTNS EQVDEDADVD TAMAALDDQP AEAQPPSPRS SSPCRTPEPD NDAHLRSSSR PCSPVHHHEG HAKLSSSPPR ASPVRMAPSY VLKKAQVLSA GSRRRKEQRY RSVISDIFDG SILSLVQCLT CDRVSTTVET FQDLSLPIPG KEDLAKLHSA IYQNVPAKPG ACGDSYAAQG WLAFIVEYIR RFVVSCTPSW FWGPVVTLED CLAAFFAADE LKGDNMYSCE RCKKLRNGVK YCKVLRLPEI LCIHLKRFRH EVMYSFKINS HVSFPLEGLD LRPFLAKECT SQITTYDLLS VICHHGTAGS GHYIAYCQNV INGQWYEFDD QYVTEVHETV VQNAEGYVLF YRKSSEEAMR ERQQVVSLAA MREPSLLRFY VSREWLNKFN TFAEPGPITN QTFLCSHGGI PPHKYHYIDD LVVILPQNVW EHLYNRFGGG PAVNHLYVCS ICQVEIEALA KRRRIEIDTF IKLNKAFQAE ESPGVIYCIS MQWFREWEAF VKGKDNEPPG PIDNSRIAQV KGSGHVQLKQ GADYGQISEE TWTYLNSLYG GGPEIAIRQS VAQPLGPENL HGEQKIEAET RAV
Predicted molecular weight104 kDa including tags
Amino acids2 to 914
TagsHis tag N-Terminus , DDDDK tag C-Terminus
Our Abpromise guarantee covers the use of ab271783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)
100ug/ml DDDDK peptide
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Deubiquitinating enzyme 20
FunctionDeubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Sequence similaritiesBelongs to the peptidase C19 family. USP20/USP33 subfamily.
Contains 2 DUSP domains.
Contains 1 UBP-type zinc finger.
DomainThe UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue.
modificationsUbiquitinated via a VHL-dependent pathway for proteasomal degradation.
Cellular localizationCytoplasm > perinuclear region. According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271783 has not yet been referenced specifically in any publications.