Product nameRecombinant Human USP25 protein
See all USP25 proteins and peptides
Protein lengthFull length protein
Amino Acid Sequence
Molecular weight124 kDa including tags
Our Abpromise guarantee covers the use of ab157024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Purity>95% by SDS-PAGE .
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.02% DTT, 0.6% Tris, 0.29% Sodium chloride
- Deubiquitinating enzyme 25
- Ubiquitin carboxyl-terminal hydrolase 25
- Ubiquitin specific peptidase 25
FunctionDeubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.
Tissue specificityIsoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific.
Sequence similaritiesBelongs to the peptidase C19 family.
Contains 1 UBA-like domain.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially by SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
Cellular localizationCytoplasm and Cytoplasm. Nucleus. Some transient punctuate nuclear location in myotubes during myocyte development.
- Information by UniProt
ab157024 has not yet been referenced specifically in any publications.