Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      124 kDa including tags

Specifications

Our Abpromise guarantee covers the use of ab157024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    >95% by SDS-PAGE .

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.6% Tris, 0.29% Sodium chloride

General Info

  • Alternative names
    • Deubiquitinating enzyme 25
    • Ubiquitin carboxyl-terminal hydrolase 25
    • Ubiquitin specific peptidase 25
    • ubiquitin specific protease 25
    • ubiquitin specific protease USP25
    • Ubiquitin thiolesterase 25
    • Ubiquitin-specific-processing protease 25
    • UBP25_HUMAN
    • USP on chromosome 21
    • USP21
    • USP25
    see all
  • Function
    Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
    The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.
  • Tissue specificity
    Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific.
  • Sequence similarities
    Belongs to the peptidase C19 family.
    Contains 1 UBA-like domain.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
  • Post-translational
    modifications
    Acetylated.
    Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially by SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
    Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
    Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
  • Cellular localization
    Cytoplasm and Cytoplasm. Nucleus. Some transient punctuate nuclear location in myotubes during myocyte development.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab157024 (1µg) stained with Coomassie.
  • DUB Assay: 50nM ab157024; 500nM Ubiquitin-AMC; 50mM HEPES, pH7.8, 0.5mM EDTA, 1mM DTT.

References

ab157024 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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