Description

  • Product name

    Recombinant Human USP33 protein
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      26 kDa
    • Amino acids

      71 to 303
    • Tags

      His-DHFR tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab127357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride

  • Reconstitution
    Reconstitute with water to desired concentration.

General Info

  • Alternative names

    • Deubiquitinating enzyme 33
    • EC 3 1 2 15
    • hVDU1
    • KIAA1097
    • MGC16868
    • OTTHUMP00000011234
    • OTTHUMP00000011330
    • OTTHUMP00000011331
    • pVHL interacting deubiquitinating enzyme 1
    • Ubiquitin carboxyl terminal hydrolase 33
    • Ubiquitin carboxyl-terminal hydrolase 33
    • Ubiquitin specific peptidase 33
    • Ubiquitin specific processing protease 33
    • ubiquitin specific protease 33
    • Ubiquitin thioesterase 33
    • Ubiquitin thiolesterase 33
    • Ubiquitin-specific-processing protease 33
    • UBP33_HUMAN
    • USP 33
    • Usp33
    • VDU 1
    • VDU1
    • VHL interacting deubiquitinating enzyme 1
    • VHL-interacting deubiquitinating enzyme 1
    see all
  • Function

    Deubiquitinating enzyme involved in various processes such as cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the peptidase C19 family. USP20/USP33 subfamily.
    Contains 2 DUSP domains.
    Contains 1 UBP-type zinc finger.
  • Domain

    The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 86 is replaced by a Glu residue.
  • Post-translational
    modifications

    Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.
  • Cellular localization

    Cytoplasm > perinuclear region. According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear.
  • Information by UniProt

References

ab127357 has not yet been referenced specifically in any publications.

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