Description

  • Product name

    Recombinant Human USP44 protein
  • Expression system

    Wheat germ
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MLAMDTCKHVGQLQLAQDHSSLNPQKWHCVDCNTTESIWACLSCSHVACG RYIEEHALKHFQESSHPVALEVNEMYVFCYLCDDYVLNDNATGDLKLLRR TLSAIKSQNYHCTTRSGRFLRSMGTGDDSYFLHDGAQSLLQSEDQLYTAL WHRRRILMGKIFRTWFEQSPIGRKKQEEPFQEKIVVKREVKKRRQELEYQ VKAELESMPPRKSLRLQGLAQSTIIEIVSVQVPAQTPASPAKDKVLSTSE NEISQKVSDSSVKRRPIVTPGVTGLRNLGNTCYMNSVLQVLSHLLIFRQC FLKLDLNQWLAMTASEKTRSCKHPPVTDTVVYQMNECQEKDTGFVCSRQS SLSSGLSGGASKGRKMELIQPKEPTSQYISLCHELHTLFQVMWSGKWALV SPFAMLHSVWRLIPAFRGYAQQDAQEFLCELLDKIQRELETTGTSLPALI PTSQRKLIKQVLNVVNNIFHGQLLSQVTCLACDNKSNTIEPFWDLSLEFP ERYQCSGKDIASQPCLVTEMLAKFTETEALEGKIYVCDQCNSKRRRFSSK PVVLTEAQKQLMICHLPQVLRLHLKRFRWSGRNNREKIGVHVGFEEILNM EPYCCRETLKSLRPECFIYDLSAVVMHHGKGFGSGHYTAYCYNSEGGFWV HCNDSKLSMCTMDEVCKAQAYILFYTQRVTENGHSKLLPPELLLGSQHPN EDADTSSNEILS
    • Amino acids

      1 to 712
    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab164494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

  • Form

    Liquid
  • Additional notes

    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • Deubiquitinating enzyme 44
    • E430004F17Rik
    • Ubiquitin carboxyl-terminal hydrolase 44
    • ubiquitin specific peptidase 44
    • Ubiquitin thiolesterase 44
    • Ubiquitin-specific-processing protease 44
    • UBP44_HUMAN
    • USP44
    see all
  • Function

    Deubiquitinase that plays a key role in the spindle checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint), thereby preventing premature activation of the APC/C.
  • Tissue specificity

    Expressed in testis.
  • Sequence similarities

    Belongs to the peptidase C19 family. USP44 subfamily.
    Contains 1 UBP-type zinc finger.
  • Developmental stage

    Elevated in mitosis; levels increase in mitotic cells and rapidly decrease once cells have completed chromosome attachment and exit from mitosis.
  • Post-translational
    modifications

    Phosphorylated.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination.
  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • ab164494 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab164494 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab164494.
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