The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
Deubiquitinating enzyme 44
Ubiquitin carboxyl-terminal hydrolase 44
ubiquitin specific peptidase 44
Ubiquitin thiolesterase 44
Ubiquitin-specific-processing protease 44
Deubiquitinase that plays a key role in the spindle checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint), thereby preventing premature activation of the APC/C.
Expressed in testis.
Belongs to the peptidase C19 family. USP44 subfamily. Contains 1 UBP-type zinc finger.
Elevated in mitosis; levels increase in mitotic cells and rapidly decrease once cells have completed chromosome attachment and exit from mitosis.
Phosphorylated. Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination.