Recombinant human USP5 protein (ab198421)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 55% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human USP5 protein
See all USP5 proteins and peptides -
Biological activity
Specific Activity: 786 pmol/min/µg
Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and ab198421 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.
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Purity
>= 55 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
AELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFL GFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAI GVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAV EALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSK CDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTI TPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMN QRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQ RKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGER VPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNC RSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEE LLEYEEKKRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQ AKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQ LRGTGLQPGEEELPDIAPPLVTPDEPKGSLGFYGNEDEDSFCSPHFSSPT SPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDF ANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNS LERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPKVRDGPGKY QLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIY FYQRVAS -
Predicted molecular weight
96 kDa including tags -
Amino acids
2 to 858 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
NM_001098536.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol, 0.05% DTT
Contains 90 µg/ml DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Deubiquitinating enzyme 5
- Isopeptidase T
- ISOT
see all -
Function
Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 2 UBA domains.
Contains 1 UBP-type zinc finger. -
Domain
The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. -
Post-translational
modificationsThe N-terminus is blocked. - Information by UniProt
Images
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SDS-PAGE - Recombinant human USP5 protein (ab198421)
10% SDS-PAGE analysis of ab198421 (2.5 μg).
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Functional Studies - Recombinant human USP5 protein (ab198421)Activity assay using ab198421.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab198421 has not yet been referenced specifically in any publications.
