Recombinant human USP5 protein (ab198421)

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Description

  • Product name

    Recombinant human USP5 protein
    See all USP5 proteins and peptides

  • Biological activity

    Specific Activity: 786 pmol/min/µg

    Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and ab198421 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.

  • Purity

    >= 55 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    P45974

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      AELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFL GFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAI GVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAV EALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSK CDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTI TPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMN QRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQ RKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGER VPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNC RSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEE LLEYEEKKRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQ AKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQ LRGTGLQPGEEELPDIAPPLVTPDEPKGSLGFYGNEDEDSFCSPHFSSPT SPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDF ANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNS LERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPKVRDGPGKY QLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIY FYQRVAS

    • Predicted molecular weight

      96 kDa including tags

    • Amino acids

      2 to 858

    • Tags

      DDDDK tag N-Terminus

    • Additional sequence information

      NM_001098536.

Specifications

Our Abpromise guarantee covers the use of ab198421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol, 0.05% DTT

    Contains 90 µg/ml DDDDK peptide.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Deubiquitinating enzyme 5
    • Isopeptidase T
    • ISOT
    • ISOT 1
    • Ubiquitin carboxyl-terminal hydrolase 5
    • Ubiquitin isopeptidase T
    • Ubiquitin specific peptidase 5
    • Ubiquitin specific peptidase 5 (isopeptidase T)
    • Ubiquitin specific processing protease 5
    • Ubiquitin specific protease 5 (isopeptidase T)
    • Ubiquitin specific protease 5 (ubiquitin isopeptidase T)
    • Ubiquitin thioesterase 5
    • Ubiquitin thiolesterase 5
    • Ubiquitin-specific-processing protease 5
    • UBP5_HUMAN
    • Usp5
    see all

  • Function

    Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

  • Sequence similarities

    Belongs to the peptidase C19 family.
    Contains 2 UBA domains.
    Contains 1 UBP-type zinc finger.

  • Domain

    The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.

  • Post-translational
    modifications

    The N-terminus is blocked.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human USP5 protein (ab198421)
    SDS-PAGE - Recombinant human USP5 protein (ab198421)

    10% SDS-PAGE analysis of ab198421 (2.5 μg).

  • Functional Studies - Recombinant human USP5 protein (ab198421)
    Functional Studies - Recombinant human USP5 protein (ab198421)

    Activity assay using ab198421.

References

ab198421 has not yet been referenced specifically in any publications.

Publishing research using ab198421? Please let us know so that we can cite the reference in this datasheet.

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