Description

  • Product name

    Recombinant Human VAMP8/EDB protein
  • Purity

    > 85 % SDS-PAGE.
    ab79141 is purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MEEASEGGGN DRVRNLQSEV EGVKNIMTQN VERILARGEN LEHLRNKTED LEATSEHFKT TSQKVARKFW WKNVKM
    • Amino acids

      1 to 76

Specifications

Our Abpromise guarantee covers the use of ab79141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Previously labelled as VAMP8. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.002% PMSF, 0.242% Tris, 50% Glycerol, 1.17% Sodium chloride

General Info

  • Alternative names

    • EDB
    • Endobrevin
    • VAMP 8
    • VAMP-8
    • VAMP8
    • VAMP8_HUMAN
    • Vesicle associated membrane protein 8
    • Vesicle-associated membrane protein 8
    see all
  • Function

    Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.
  • Tissue specificity

    Platelets.
  • Sequence similarities

    Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab79141 at approximately 11kDa (3µg).

References

ab79141 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

I am sorry for the delay in getting back to you. I can now confirm that the new batch of VAMP8 protein (ab79141) has been produced.

Before we arrange for the shipping I would just like to confirm if you are happy with the product. The production has not been as pure as we had hoped. Although the lab are constantly trying to improve on the amount of degradation observed with this particular protein by using different buffers there is still a small amount of the second band observed in the original lot. This is however much less prominent that what you observed in your gel.

I have attached the SDS-PAGE gel of the new batch for your consideration. If you would like for me to arrange for this to be sent to you please let me know. Otherwise, I can arrange for a credit note or refund.

I look forward to receiving your reply.

Read More

Answer

Thank you for your reply.

The new batch is expected to be ready in 2-3 weeks time. It will be assessed by SDS-PAGE to assess its purity before going into stock. I am sorry for this delay and any inconvenience it may cause you.

I look forward to receiving your reply.

Read More

Answer

I am sorry for the delay in getting back to you.

Following the concerns you have raised against the VAMP8 protein (ab79141) we have retested the protein to see if we could identify the same two bands you have observed. When observed by SDS-PAGE we did observe a band below the expected band (˜12 kDa), please refer to the attached image. This was not as stark as you had observed but none the less this side product was observed. It therefore appears that there may have been some degradation of the protein following its initial purification. We are looking into this in more detail to prevent this from occurring in the future.

I am sorry for the inconvenience this problem has caused you. If you would like I can offer you a replacement vial of ab79141 of a newly produced batch. Or if you would prefer I can offer a credit note or refund.

I look forward to receiving your reply.

Read More

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