Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MAKVEQVLSL EPQHELKFRG PFTDVVTTNL KLGNPTDRNV CFKVKTTAPR RYCVRPNSGI IDAGASINVS VMLQPFDYDP NEKSKHKFMV QSMFAPTDTS DMEAVWKEAK PEDLMDSKLR CVFELPAEND KP
    • Molecular weight

      16 kDa including tags
    • Amino acids

      1 to 132
    • Tags

      His tag C-Terminus
    • Additional sequence information

      (NP_004729).

Specifications

Our Abpromise guarantee covers the use of ab220445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level

    < 1.000 Eu/µg
  • Purity

    > 95 % SDS-PAGE.

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 95% PBS, 5% Trehalose

    Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • ALS 8
    • ALS8
    • D2Abb2e
    • UNQ484/PRO983
    • Vamp 33b
    • VAMP associated 33 kDa protein
    • VAMP associated protein B
    • VAMP associated protein B and C
    • VAMP associated protein B/C
    • VAMP associated protein C
    • VAMP B
    • VAMP B VAMP C
    • VAMP B/VAMP C
    • VAMP C
    • VAMP vesicle associated membrane protein associated protein B and C
    • Vamp33b
    • VAMPB
    • VAMPB/VAMPC
    • VAMPC
    • VAP 33b
    • VAP B
    • VAP B/VAP C
    • VAP C
    • VAP33b
    • VAPB/VAPC
    • VAPC
    • Vesicle associated membrane protein associated protein B and C
    • Vesicle associated membrane protein associated protein B/C
    see all
  • Relevance

    VAPB contains 1 MSP domain and it may play a role in vesicle trafficking. Defects in VAPB are a cause of proximal adult autosomal dominant spinal muscular atrophy [MIM:182980]; also called late onset spinal muscular atrophy Finkel type. Spinal muscular atrophies are neurodegenerative disorders characterized by degeneration of lower motor neurons, leading to progressive paralysis muscular atrophy. This form is a late adult onset form of the disease (after age 20 years). The patients show a benign course, most of them remaining ambulatory 10 to 40 years after clinical onset.
  • Cellular localization

    Cell membrane; Single-pass type IV membrane. Intracytoplasmic membrane; Single-pass type IV membrane protein. Note: Present in the plasma membrane and in intracellular vesicles.

Images

  • SDS-PAGE analysis of reduced ab220445 stained overnight with Coomassie Blue.

    DTT-reduced Protein migrates as 16-18 kDa in SDS-PAGE .

References

ab220445 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab220445.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up