Description

  • Product name

    Recombinant human VCAM1 protein
    See all VCAM1 proteins and peptides
  • Biological activity

    Determined by its ability to support the adhesion of human U937 cells. The expected ED50 for this effect is 0.8-1.0 μg/mL.

  • Purity

    >= 98 % SDS-PAGE.
    assessed also by HPLC analysis
  • Endotoxin level

    < 0.100 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      FKIETTPESR YLAQIGDSVS LTCSTTGCES PFFSWRTQID SPLNGKVTNE GTTSTLTMNP VSFGNEHSYL CTATCESRKL EKGIQVEIYS FPKDPEIHLS GPLEAGKPIT VKCSVADVYP FDRLEIDLLK GDHLMKSQEF LEDADRKSLE TKSLEVTFTP VIEDIGKVLV CRAKLHIDEM DSVPTVRQAV KELQVYISPK NTVISVNPST KLQEGGSVTM TCSSEGLPAP EIFWSKKLDN GNLQHLSGNA TLTLIAMRME DSGIYVCEGV NLIGKNRKEV ELIVQEKPFT VEISPGPRIA AQIGDSVMLT CSVMGCESPS FSWRTQIDSP LSGKVRSEGT NSTLTLSPVS FENEHSYLCT VTCGHKKLEK GIQVELYSFP RDPEIEMSGG LVNGSSVTVS CKVPSVYPLD RLEIELLKGE TILENIEFLE DTDMKSLENK SLEMTFIPTI EDTGKALVCQ AKLHIDDMEF EPKQRQSTQT LYVNVAPRDT TVLVSPSSIL EEGSSVNMTC LSQGFPAPKI LWSRQLPNGE LQPLSENATL TLISTKMEDS GVYLCEGINQ AGRSRKEVEL IIQVTPKDIK LTAFPSESVK EGDTVIISCT CGNVPETWII LKKKAETGDT VLKSIDGAYT IRKAQLKDAG VYECESKNKV GSQLRSLTLD VQGRENNKDY FSP
    • Predicted molecular weight

      74 kDa
    • Amino acids

      25 to 697
    • Additional sequence information

      Extracellular domain.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab194900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    pH: 7.00
    Constituent: 0.16% Sodium phosphate

    lyophilized from a filter sterilized solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.

General Info

  • Alternative names

    • CD106
    • CD106 Antigen
    • INCAM 100
    • INCAM-100
    • L1CAM
    • MGC99561
    • V-CAM 1
    • Vascular Cell Adhesion Molecule 1
    • Vascular cell adhesion protein 1
    • VCAM 1
    • VCAM-1
    • VCAM1
    • VCAM1_HUMAN
    see all
  • Function

    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificity

    Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similarities

    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications

    Sialoglycoprotein.
  • Cellular localization

    Membrane.
  • Information by UniProt

References

ab194900 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab194900.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up