The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Determined by its ability to support the adhesion of human U937 cells. The expected ED50 for this effect is 0.8-1.0 μg/mL.
< 0.100 Eu/µg
% SDS-PAGE. assessed also by HPLC analysis
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
pH: 7.00 Constituent: 0.16% Sodium phosphate
lyophilized from a filter sterilized solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.
Vascular Cell Adhesion Molecule 1
Vascular cell adhesion protein 1
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.