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  1. Link

    recombinant-human-vcp-protein-active-ab269130.pdf

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Recombinant human VCP protein (Active) (ab269130)

  • Datasheet
  • SDS
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Functional Studies - Recombinant human VCP protein (Tagged) (ab269130)
  • SDS-PAGE - Recombinant human VCP protein (Tagged) (ab269130)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 85% SDS-PAGE
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

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Description

  • Product name

    Recombinant human VCP protein (Active)
    See all VCP proteins and peptides
  • Biological activity

    ab269130 specific activity was determined to be 140 nmol/min/mg in a proprietary ATPase assay.

  • Purity

    > 85 % SDS-PAGE.
    Affinity purified.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    P55072
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Molecular weight information

      Approx 120 kDa by SDS-PAGE
    • Tags

      GST tag N-Terminus
    • Additional sequence information

      NM_007126

Associated products

  • Related Products

    • Anti-GST antibody [EPR4236] (ab111947)
    • Anti-GST antibody (ab9085)

Specifications

Our Abpromise guarantee covers the use of ab269130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 15S Mg(2+) ATPase p97 subunit
    • 15S Mg(2+)-ATPase p97 subunit
    • ALS14
    • ATPase p97
    • CDC48
    • IBMPFD
    • MGC131997
    • MGC148092
    • MGC8560
    • p97
    • TER ATPase
    • TERA
    • TERA_HUMAN
    • Transitional endoplasmic reticulum ATPase
    • Valosin containing protein
    • Valosin-containing protein
    • VCP
    • Yeast Cdc48p homolog
    see all
  • Function

    Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A.
  • Involvement in disease

    Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) [MIM:167320]; also known as muscular dystrophy, limb-girdle, with Paget disease of bone or pagetoid amyotrophic lateral sclerosis or pagetoid neuroskeletal syndrome or lower motor neuron degeneration with Paget-like bone disease. IBMPFD features adult-onset proximal and distal muscle weakness (clinically resembling limb girdle muscular dystrophy), early-onset Paget disease of bone in most cases and premature frontotemporal dementia.
  • Sequence similarities

    Belongs to the AAA ATPase family.
  • Post-translational
    modifications

    Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
  • Target information above from: UniProt accession P55072 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human VCP protein (Tagged) (ab269130)
    Functional Studies - Recombinant human VCP protein (Tagged) (ab269130)

    ab269130 specific activity was determined to be 140 nmol/min/mg in a proprietary ATPase assay.

  • SDS-PAGE - Recombinant human VCP protein (Tagged) (ab269130)
    SDS-PAGE - Recombinant human VCP protein (Tagged) (ab269130)

    SDS-PAGE analysis of ab269130.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab269130? Please let us know so that we can cite the reference in this datasheet.

ab269130 has not yet been referenced specifically in any publications.

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