Recombinant human VEGF 121B protein (ab61872)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human VEGF 121B protein
See all VEGF 121B proteins and peptides -
Biological activity
Biological Activity: The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.2-0.4 ng/ml. -
Purity
> 95 % SDS-PAGE.
Purity greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab61872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Additional notes
Aliquot and freeze at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized VEGF in sterile H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
General Info
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Alternative names
- MVCD1
- Vascular endothelial growth factor A
- Vascular permeability factor
see all -
Relevance
VEGFA is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. VEGF 121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates. -
Cellular localization
Secreted
Images
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Functional Studies - Recombinant human VEGF 121B protein (ab61872)Functional analysis of ab61872
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SDS-PAGE - Recombinant human VEGF 121B protein (ab61872)SDS PAGE analysis of ab61872 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
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Functional Studies - Recombinant human VEGF 121B protein (ab61872)ab61872 used in Functional Studies.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab61872 has not yet been referenced specifically in any publications.
