Recombinant human VEGF 121B protein (ab61872)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R

Specifications

Our Abpromise guarantee covers the use of ab61872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Biological Activity: The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.2-0.4 ng/ml.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 95 % SDS-PAGE.
    Purity greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
  • Form
    Lyophilised
  • Additional notes
    Aliquot and freeze at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized VEGF in sterile H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • MVCD1
    • Vascular endothelial growth factor A
    • Vascular permeability factor
    • VEGF
    • VEGF A
    • VPF
    see all
  • Relevance
    VEGFA is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. VEGF 121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.
  • Cellular localization
    Secreted

Images

  • Other - Recombinant human VEGF 121B protein (ab61872)
    Other - Recombinant human VEGF 121B protein (ab61872)
    ab61872 used in Functional Studies.

References

ab61872 has not yet been referenced specifically in any publications.

Publishing research using ab61872? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

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Votes: Highest First

Hello,



I recently purchased two VEGF proteins, products:



ab61861

ab61872



The product literature that came with them each has a graph showing the amount of proliferation of HUVECs when su...

Read More

Hello!

Unfortunately we don't have the graphs saved in another format than the one on the datasheet, but I can list the concentrations below.

ab61861

Human VEGF-165 ng/ml [log scale] .001, .01, .1, 1.0, 10.0, 100.0

H...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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