Recombinant human VEGF 165A protein (Active) (ab259412)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, MS, Functional Studies
Description
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Product name
Recombinant human VEGF 165A protein (Active)
See all VEGF 165A proteins and peptides -
Biological activity
Fully biologically active determined by the dose dependent increase in proliferation of Human umbilical vein endothelial cells.
ED50 is ≤ 5.3 ng/mL, corresponding to a specific activity of 0.19 x 106 units/mg.
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Purity
>= 95 % SDS-PAGE.
Purity =95% by HPLC -
Endotoxin level
<=0.005 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Carrier free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR -
Predicted molecular weight
19 kDa -
Molecular weight information
Predicted mass is 19223.56 (+/- 10 Da by ESI-TOF). Mass at 19066.94 is due to loss of arginine on the C-terminus; peak at 19099.52 is a process related impurity. -
Amino acids
27 to 191 -
Additional sequence information
N-terminal glycine tag. Full-length mature chain lacking the signal peptide. Isoform VEGF165
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Specifications
Our Abpromise guarantee covers the use of ab259412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
HPLC
Mass Spectrometry
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate
Buffer lyophlized fromThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
General Info
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Alternative names
- glioma-derived endothelial cell mitogen
- MGC70609
- MVCD1
see all -
Function
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
Tissue specificity
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
Involvement in disease
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Cellular localization
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
Images
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Functional Studies - Recombinant human VEGFA protein (Active) (ab259412)
Fully biologically active determined by the dose dependent increase in proliferation of Human umbilical vein endothelial cells.
ED50 is ≤ 5.3 ng/mL, corresponding to a specific activity of 0.19 x 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3373539-2
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SDS-PAGE - Recombinant human VEGFA protein (Active) (ab259412)SDS-PAGE analysis of ab259412.
Protein migrates higher than expected due to glycosylation.
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Mass Spectrometry - Recombinant human VEGFA protein (Active) (ab259412)Mass determination by ESI TOF.
Predicted mass is 19223.56 (+/- 10 Da by ESI-TOF). Mass at 19066.94 is due to loss of arginine on the C-terminus; peak at 19099.52 is a process related impurity.
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HPLC - Recombinant human VEGFA protein (Active) (ab259412)HPLC analysis of ab259412.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab259412 has not yet been referenced specifically in any publications.
