Recombinant human VEGF 165A protein (Active) (ab9571)

Submit an Abreview Q&A (8)Specific References (4)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      P15692-4
    • Species
      Human
    • Sequence
      APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
    • Amino acids
      27 to 191
    • Additional sequence information
      Recombinant human VEGF165 is a 38.2 kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains. It is the full length mature protein minus the signal peptide.

Specifications

Our Abpromise guarantee covers the use of ab9571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-8.0 ng/ml.

  • Applications

    Cell differentiation

    Cellular Activation

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 98 % n/a.
    Sterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Form
    Lyophilised
  • Additional notes
    The biological activity of this product is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-8.0 ng/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in 100 µl dH2O

General Info

  • Alternative names
    • glioma-derived endothelial cell mitogen
    • MGC70609
    • MVCD1
    • Vascular Endothelial Growth Factor
    • Vascular endothelial growth factor A
    • Vascular permeability factor
    • VEGF
    • VEGF-A
    • VEGF165
    • Vegfa
    • VEGFA_HUMAN
    • VPF
    see all
  • Function
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease
    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization
    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt

Images

  • Cell differentiation - Recombinant human VEGF 165A protein (Active) (ab9571)
    Cell differentiation - Recombinant human VEGF 165A protein (Active) (ab9571)This image is courtesy of Sun Yung, Centro de Investigación Príncipe Felipe.

    Human embryonic stem cells (H9) were differentiated both as embryoid bodies and monolayer cultures (on Matrigel) using a protocol described in Kennedy et al., 2006. BMP-4, FGF-basic (ab9596) and VEGF (ab9571) were sequentially added to the media to direct differentiation. These images were taken at day 8, where cell adhesion antigens CD31 (ab32457 in green) and CD34 (ab8536 in red) are abundant and represent primitive haematopoietic cells within the culture. DAPI staining is shown in blue.

References

This product has been referenced in:
  • Jiménez-Torres JA  et al. LumeNEXT: A Practical Method to Pattern Luminal Structures in ECM Gels. Adv Healthc Mater 5:198-204 (2016). Read more (PubMed: 26610188) »
  • Tan J  et al. Extracts of endophytic fungus xkc-s03 from Prunella vulgaris L. spica inhibit gastric cancer in vitro and in vivo. Oncol Lett 9:945-949 (2015). Read more (PubMed: 25624914) »

See all 4 Publications for this product

Publishing research using ab9571? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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8 reviews or Q&A

10
Votes: Highest First

I have a scaffold that contains sulfated glycoaminoglycans (GAGs). GAGs can bind VEGF in vivo. I need to absorb VEGF to these scaffolds so the first question is if VEGF ab9571 binds to GAGs in its form (Is it a dimer? Does it have the GAG attachment ci...

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The Active human VEGF full length protein (ab9571) has been tested on HUVECs and is known to stimulate proliferation of these cells, which shows that the GAG sites are present and active.
The recombinant human VEGF165 is a 38.2 kDa disulfide-linke...

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Dear All,



Are those proteins below injectable in Mice?




1. SPARC protein (MBP) (ab92965)



2. TGF beta 1 protein (Active) (ab50036)




3. VEGF protein (Active) (ab9571)

Thank you for contacting us and for your patience.

I had to contact the laboratory in order to get more information about the use of these three proteins.

I can confirm, that for the ab92965 SPARC protein (MBP) to our knowledge it ha...

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Can you please send me the sequences for these VEGF proteins?

Thank you for contacting us. The sequences are as follows:
ab9571: two polypeptide chains with the sequence APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYI FKPS CVPLMRCGGCCNDEGLECVP TEESNITMQI MRI KPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKH...

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Dear technical team,

Our customer would like to purchase VEGF protein and he has CST #8065.

http://www.cellsignal.com/products/8065.html

I searched alternative item ab9571. It is also 165 amino acids but there's not det...

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Thank you for contacting us and your interest in our products.

We have a few different products which may be of interest to your customer. Proteins ab61861, ab56620 and ab83572 are all of the VEGF 165A form. Additionally, ab61861 and ab83572 ...

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I am interested in VEGF165 (recombinant expressed in E. coli) as well as in an Anti-VEGF-antibody. I would like to do conformation analysis and I need the mature VEGF-protein with length of 165 amino acids. So there should be no N-terminal signal seque...

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Thank you for contacting us.

For the proteins, I would suggest using ab61861 as this protein is arecombinant Human VEGF 165A protein (double, non-glycosylated, polypeptide chain containing 165 amino acids, MW 38kDa, expressed in E.coli). Read More

Need a antibody a protein match pair for VEGF

Thank you for contacting us today. I can confirm that the VEGF protein ab9571 has been used in ELISA with the VEGF antibody ab9570. Therefore they are a suitable match and ab9571 can be used to generate a standard curve for ELISA. Please let me ...

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I have a new antibody for pVEGFR2. I wanted to grow HUVEC cells as a positive control for this antibody. To do so I believe I need to add VEGF to stimulate growth. If I added this VEGF protein to HUVEC cells in culture an I correct in thinki8ng that it...

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Thank you for your enquiry. Please see the response from the originator of the recombinant protein: Regarding our human VEGF, we test this by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) us...

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How should this protein be reconstituted? How pure is it?

Thank you very much for your enquiry and patience. Ab9571 should be reconstituted with water to a concentration of 0.1-1.0 mg/ml. Also, this product was sterile filtered and it is greater than 98% pure by SDS-PAGE and HPLC analyses. Please con...

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