Recombinant human VEGF Receptor 1 protein (Active) (ab216987)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human VEGF Receptor 1 protein (Active)
See all VEGF Receptor 1 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 at 2 μg/mL can bind ab216987 with a linear range of 3-50 ng/mL.
Measured by its ability to bind Human VEGF165 in the SPR assay with an estimated KD of 0.1nM.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKESERLSI TKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESAIYI FISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLDT LIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHRQT NTIIDVQISTPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRA SVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSV HIYDKAFITVKHRKQQVLETVAGKRSYRLSMKVKAFPSPEVVWLKDGLPA TEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQSNVFKNLTATLIVNV KPQIYEKAVSSFPDPALYPLGSRQILTCTAYGIPQPTIKWFWHPCNHNHS EARCDFCSNNEESFILDADSNMGNRIESITQRMAIIEGKNKMASTLVVAD SRISGIYICIASNKVGTVGRNISFYITDVPNGFHVNLEKM PTEGEDLK LSCTVNKFLYRDVTWILLRTVNNRTMHYSISKQKMAITKEHSITLNLTIM NVSLQDSGTYACRARNVYTGEEILQKKEITIRDQEAPYLLRNLSDHTVAI SSSTTLDCHANGVPEPQITWFKNNHKIQQEPGIILGPGSSTLFIERVTEE DEGVYHCKATNQKGSVESSAYLTVQGTSDKSN -
Predicted molecular weight
83 kDa including tags -
Amino acids
27 to 756 -
Tags
His tag C-Terminus -
Additional sequence information
NP_002010.1.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab216987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 97% PBS
Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
General Info
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Alternative names
- EC 2.7.10.1
- FLT
- FLT 1
see all -
Function
Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis. -
Tissue specificity
Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
Images
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Immobilized Human VEGF165, Tag Free (ab155740) at 0.01 μg/mL (100 μL/well) can bind ab216987 with a linear range of 4-31 ng/mL
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SDS-PAGE analysis of reduced ab216987 stained overnight with Coomassie Blue.
Note: The reducing protein migrates as 115-125 kDa on a SDS-PAGE gel under reducing condition due to glycosylation..
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab216987 has not yet been referenced specifically in any publications.