Bioactive grade

Recombinant human VEGF Receptor 1 protein (Active) (ab216987)

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Description

  • Product name

    Recombinant human VEGF Receptor 1 protein (Active)
    See all VEGF Receptor 1 proteins and peptides

  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 at 2 μg/mL can bind ab216987 with a linear range of 3-50 ng/mL.

    Measured by its ability to bind Human VEGF165  in the SPR assay with an estimated KD of 0.1nM.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P17948

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKESERLSI TKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESAIYI FISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLDT LIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHRQT NTIIDVQISTPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRA SVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSV HIYDKAFITVKHRKQQVLETVAGKRSYRLSMKVKAFPSPEVVWLKDGLPA TEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQSNVFKNLTATLIVNV KPQIYEKAVSSFPDPALYPLGSRQILTCTAYGIPQPTIKWFWHPCNHNHS EARCDFCSNNEESFILDADSNMGNRIESITQRMAIIEGKNKMASTLVVAD SRISGIYICIASNKVGTVGRNISFYITDVPNGFHVNLEKM PTEGEDLK LSCTVNKFLYRDVTWILLRTVNNRTMHYSISKQKMAITKEHSITLNLTIM NVSLQDSGTYACRARNVYTGEEILQKKEITIRDQEAPYLLRNLSDHTVAI SSSTTLDCHANGVPEPQITWFKNNHKIQQEPGIILGPGSSTLFIERVTEE DEGVYHCKATNQKGSVESSAYLTVQGTSDKSN

    • Predicted molecular weight

      83 kDa including tags

    • Amino acids

      27 to 756

    • Tags

      His tag C-Terminus

    • Additional sequence information

      NP_002010.1.

Specifications

Our Abpromise guarantee covers the use of ab216987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 97% PBS

    Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage. After reconstitution under sterile conditions for 3 months (-70°C).

General Info

  • Alternative names

    • EC 2.7.10.1
    • FLT
    • FLT 1
    • Flt-1
    • FLT1
    • Fms like tyrosine kinase 1
    • Fms related tyrosine kinase 1
    • Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
    • Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor
    • Fms-like tyrosine kinase 1
    • FRT
    • Soluble VEGF receptor 1 14
    • Soluble VEGFR1 variant 2
    • Soluble VEGFR1 variant 21
    • Tyrosine protein kinase FRT
    • Tyrosine protein kinase receptor FLT
    • Tyrosine-protein kinase FRT
    • Tyrosine-protein kinase receptor FLT
    • Vascular endothelial growth factor receptor 1
    • Vascular endothelial growth factor vascular permeability factor receptor
    • Vascular permeability factor receptor
    • Vascular permeability factor receptor 1
    • VEGFR 1
    • VEGFR-1
    • VEGFR1
    • VGFR1_HUMAN
    see all

  • Function

    Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.

  • Tissue specificity

    Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human VEGF Receptor 1 protein (Active) (ab216987)
    Functional Studies - Recombinant human VEGF Receptor 1 protein (Active) (ab216987)

    Immobilized Human VEGF165, Tag Free (ab155740) at 0.01 μg/mL (100 μL/well) can bind ab216987 with a linear range of 4-31 ng/mL

  • SDS-PAGE - Recombinant human VEGF Receptor 1 protein (Active) (ab216987)
    SDS-PAGE - Recombinant human VEGF Receptor 1 protein (Active) (ab216987)

    SDS-PAGE analysis of reduced ab216987 stained overnight with Coomassie Blue.

    Note: The reducing protein migrates as 115-125 kDa on a SDS-PAGE gel under reducing condition due to glycosylation..

References

ab216987 has not yet been referenced specifically in any publications.

Publishing research using ab216987? Please let us know so that we can cite the reference in this datasheet.

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