Recombinant human VEGF Receptor 3 protein (Active) (ab219663)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
-
Product name
Recombinant human VEGF Receptor 3 protein (Active)
See all VEGF Receptor 3 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Human VEGF-C, His Tag at 5 μg/mL (100 μL/well) can bind ab219663 with a linear range of 0.6-10 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized ab219663 at 5 μg/mL (100 μL/well) can bind Human VEGF-D, His Tag, Strep Tag with a linear range of 0.08-0.6 μg/mL.
-
Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Predicted molecular weight
111 kDa including tags -
Amino acids
25 to 776 -
Tags
Fc tag C-Terminus -
Additional sequence information
Extracellular domain. This protein carries a human IgG1 Fc tag at the C-terminus.
-
Specifications
Our Abpromise guarantee covers the use of ab219663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
Functional Studies
-
Form
Lyophilized -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, 0.44% L-Arginine, 0.87% Sodium chloride
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
General Info
-
Alternative names
- EC 2.7.10.1
- flt 4
- FLT-4
see all -
Function
Receptor for VEGFC. Has a tyrosine-protein kinase activity. -
Tissue specificity
Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain. -
Involvement in disease
Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
Images
-
ab219663 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
The gel was stained overnight with Coomassie Blue.
The purity of the protein is greater than 95%.
The protein has a calculated MW of 111.2 kDa. As a result of glycosylation, the protein migrates as 140-160 kDa under reducing (R) conditions and 280-320 kDa under non-reducing (NR) conditions.
-
Immobilized Human VEGF-C, His Tag at 5 μg/mL (100 μL/well) can bind ab219663 with a linear range of 0.6-10 ng/mL.
-
Immobilized ab219663 at 5 μg/mL (100 μL/well) can bind Human VEGF-D, His Tag, Strep Tag with a linear range of 0.08-0.6 μg/mL.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab219663 has not yet been referenced specifically in any publications.