Bioactive grade

Recombinant human VEGF Receptor 3 protein (Active) (ab219663)

Description

  • Product name

    Recombinant human VEGF Receptor 3 protein (Active)
    See all VEGF Receptor 3 proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilized Human VEGF-C, His Tag at 5 μg/mL (100 μL/well) can bind ab219663 with a linear range of 0.6-10 ng/mL.

    Measured by its binding ability in a functional ELISA. Immobilized ab219663 at 5 μg/mL (100 μL/well) can bind Human VEGF-D, His Tag, Strep Tag with a linear range of 0.08-0.6 μg/mL.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      111 kDa including tags
    • Amino acids

      25 to 776
    • Additional sequence information

      Extracellular domain. This protein carries a human IgG1 Fc tag at the C-terminus.

Specifications

Our Abpromise guarantee covers the use of ab219663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, 0.44% L-Arginine, 0.87% Sodium chloride

    Lyophilized from 0.22 µm filtered solution.
    5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in 100 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • EC 2.7.10.1
    • flt 4
    • FLT-4
    • FLT4
    • FLT41
    • Fms related tyrosine kinase 4
    • Fms-like tyrosine kinase 4
    • LMPH1A
    • PCL
    • Soluble VEGFR3 variant 1
    • Soluble VEGFR3 variant 2
    • Soluble VEGFR3 variant 3
    • Tyrosine protein kinase receptor FLT4
    • Tyrosine-protein kinase receptor FLT4
    • Vascular endothelial growth factor receptor 3
    • Vascular endothelial growth factor receptor 3 precursor
    • VEGF R3
    • VEGFR 3
    • VEGFR-3
    • VEGFR3
    • VGFR3_HUMAN
    see all
  • Function

    Receptor for VEGFC. Has a tyrosine-protein kinase activity.
  • Tissue specificity

    Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.
  • Involvement in disease

    Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
    Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • ab219663 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions.

    The gel was stained overnight with Coomassie Blue.

    The purity of the protein is greater than 95%.

    The protein has a calculated MW of 111.2 kDa. As a result of glycosylation, the protein migrates as 140-160 kDa under reducing (R) conditions and 280-320 kDa under non-reducing (NR) conditions.

  • Immobilized Human VEGF-C, His Tag at 5 μg/mL (100 μL/well) can bind ab219663 with a linear range of 0.6-10 ng/mL.

  • Immobilized ab219663 at 5 μg/mL (100 μL/well) can bind Human VEGF-D, His Tag, Strep Tag with a linear range of 0.08-0.6 μg/mL.

References

ab219663 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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