Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human VEGF Receptor 3 protein (Active)
See all VEGF Receptor 3 proteins and peptides
Measured by its binding ability in a functional ELISA. Immobilized Human VEGF-C, His Tag at 5 μg/mL (100 μL/well) can bind ab219663 with a linear range of 0.6-10 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized ab219663 at 5 μg/mL (100 μL/well) can bind Human VEGF-D, His Tag, Strep Tag with a linear range of 0.08-0.6 μg/mL.
Purity> 95 % SDS-PAGE.
Endotoxin level< 1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthProtein fragment
Predicted molecular weight111 kDa including tags
Amino acids25 to 776
Additional sequence informationExtracellular domain. This protein carries a human IgG1 Fc tag at the C-terminus.
Our Abpromise guarantee covers the use of ab219663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, 0.44% L-Arginine, 0.87% Sodium chloride
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
- EC 220.127.116.11
- flt 4
FunctionReceptor for VEGFC. Has a tyrosine-protein kinase activity.
Tissue specificityPlacenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.
Involvement in diseaseDefects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
- Information by UniProt
ab219663 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
The gel was stained overnight with Coomassie Blue.
The purity of the protein is greater than 95%.
The protein has a calculated MW of 111.2 kDa. As a result of glycosylation, the protein migrates as 140-160 kDa under reducing (R) conditions and 280-320 kDa under non-reducing (NR) conditions.
Immobilized Human VEGF-C, His Tag at 5 μg/mL (100 μL/well) can bind ab219663 with a linear range of 0.6-10 ng/mL.
Immobilized ab219663 at 5 μg/mL (100 μL/well) can bind Human VEGF-D, His Tag, Strep Tag with a linear range of 0.08-0.6 μg/mL.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab219663 has not yet been referenced specifically in any publications.