Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Amino acids
      5 to 169
    • Tags
      His tag N-Terminus
    • Additional sequence information
      This recombinant VEGF protein comprises a 165 amino acid fragment (5-169) corresponding to the VEGF 165 isoform and is expressed in E. coli with an aminoterminal hexahistidine tag.

Specifications

Our Abpromise guarantee covers the use of ab55566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 20mM Tris, pH 8

General Info

  • Alternative names
    • Folliculostellate cell-derived growth factor
    • Glioma-derived endothelial cell mitogen
    • MGC70609
    • MVCD1
    • vascular endothelial growth factor
    • Vascular endothelial growth factor A
    • vascular endothelial growth factor A121
    • vascular endothelial growth factor A165
    • Vascular permeability factor
    • Vegf
    • VEGF A
    • VEGF-A
    • VEGF120
    • Vegfa
    • VEGFA_HUMAN
    • VPF
    see all
  • Function
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease
    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization
    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human VEGFA protein (ab55566)
    SDS-PAGE - Recombinant Human VEGFA protein (ab55566)
    ab55566 on SDS-PAGE

References

This product has been referenced in:
  • Hunter RK  et al. Adipose-Derived Stromal Vascular Fraction Cell Effects on a Rodent Model of Thin Endometrium. PLoS One 10:e0144823 (2015). WB . Read more (PubMed: 26657744) »
  • Mannell HK  et al. ARNO regulates VEGF-dependent tissue responses by stabilizing endothelial VEGFR-2 surface expression. Cardiovasc Res 93:111-9 (2012). Read more (PubMed: 22002459) »

See all 2 Publications for this product

Publishing research using ab55566? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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7 reviews or Q&A

10
Votes: Highest First

If the notes refer to the full length of protein, what the phosphorylation status of this product ab42614?

I am sorry my previous email was unclear.

The phosphorylation status of ab42614 is no known. This is not something that is determined during the production process.

I hope this information is helpful.



Thank you very much for your great suggestion. This product is perfect except the Agarose beads. Because we will use the centrifuge to separate the particle, the

rigidity ofAgarose may be not enough. However, I got very useful inf...

Read More

Thank you for your reply.

Actually it is fine to centrifuge agarose beads. Typically for IP I have centrifuged agarose beads at 500xg for 3-5 min at 4 degrees C.

Regarding your questions 1 and 2, those notes on the datasheet for ab4...

Read More

Sorry to interrupt you again. I really got some further questions.



Actually, I want to choose an Anti-GST antibody as a “bait” to fish out the GST-tagged VEGFR. For the bait, I hope to immobilize the antibody to some ...

Read More

Thank you for your enquiry.

I think I have found a product that will suit your needs:

https://www.abcam.com/GST-antibody-Agarose-ab90667.html

This is anti-GST antibody covalently linked to agarose beads. Using this product...

Read More


Thanks a lot for your useful information. I appreciate it very much.

I'm happy to help! Please contact me again if you need anything further.

Hi,



I’m sorry to interrupt you. I have some questions about your product.



We just bought a VEGF Receptor 2 protein (Tagged) (ab42614) from your company. However, we can’t find the Tag informatio...

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Thank you for your enquiry.

VEGF Receptor 2 protein (Tagged) (ab42614) contains an N-terminal GST tag.

I hope this is helpful. Please contact me again if you have any further questions.

Yes, let’s go ahead with this.


I am very pleased to hear you would like to accept our offer and test ab55566 inFunctional Studies.This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an Abreview for Functional Studiesand include...

Read More

Does this protein have any functional activity?

Thank you very much for your interest in ab55566.

To our knowledge, ab55566 has not been tested in functional assays. Therefore, I can offer a discount off a future purchase if you buy ab55566 now, test it in a functional assayand submit fee...

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