Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
      P49767
    • Species
      Human
    • Sequence
      TEETIKFAAAHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGVATNTFF KPPCVSVYRCGGCCNSEGLQCMNTSTSYLSKTLFEITVPLSQGPKPVTIS FANHTSCRCMSKLDVYRQVHSIIRR
    • Molecular weight
      15 kDa including tags
    • Amino acids
      103 to 227
    • Tags
      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab155739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. The ED50 for this effect is typically 0.15-0.7 µg/ml.
  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    > 97 % SDS-PAGE.

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 95% PBS, 5% Trehalose
    Note: Normally Mannitol or Trehalose are added as protectants before lyophilisation.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Flt 4L
    • Flt4 ligand
    • FLT4 ligand DHM
    • Flt4-L
    • Flt4L
    • Vascular endothelial growth factor C
    • Vascular endothelial growth factor related protein
    • Vascular endothelial growth factor-related protein
    • VEGF C
    • VEGF-C
    • Vegfc
    • VEGFC_HUMAN
    • VRP
    see all
  • Function
    Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
  • Tissue specificity
    Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Post-translational
    modifications
    Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing an heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - VEGFC protein (Active) (ab155739)
    SDS-PAGE - VEGFC protein (Active) (ab155739)
    SDS-PAGE analysis of reduced ab155739 stained overnight with Coomassie Blue.

References

ab155739 has not yet been referenced specifically in any publications.

Publishing research using ab155739? Please let us know so that we can cite the reference in this datasheet.

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