Description

  • Product name

    Recombinant Human Visfatin protein
    See all Visfatin proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    Purity: greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Endotoxin level

    < 0.100 Eu/µg
  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH

Associated products

Specifications

Our Abpromise guarantee covers the use of ab50134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituent: 0.036% Hydrochloric acid

  • Reconstitution
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. Note: Due to solubility reasons the protein should be kept at low pH. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

General Info

  • Alternative names

    • 1110035O14Rik
    • AI314458
    • AI480535
    • DKFZP666B131
    • EC 2.4.2.12
    • MGC117256
    • NAmPRTase
    • Nampt
    • NAMPT_HUMAN
    • Nicotinamide phosphoribosyltransferase
    • PBEF
    • PBEF 1
    • PBEF1
    • Pre B cell colony enhancing factor
    • Pre B cell colony enhancing factor 1
    • Pre B cell enhancing factor
    • Pre-B cell-enhancing factor
    • Pre-B-cell colony-enhancing factor 1
    • VF
    • Visfatin
    see all
  • Function

    Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
  • Tissue specificity

    Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
  • Pathway

    Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
  • Sequence similarities

    Belongs to the NAPRTase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt

References

ab50134 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your email. The protein we used as immunogen to produce ab113615 is ab50134; https://www.abcam.com/PBEF-protein-ab50134.html The un-conjugated version of ab113615 is ab113660 if in case the customer is interested in buying. I hope this will help!

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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