Recombinant Human VPS29 protein (ab124582)

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SDS-PAGE - Recombinant Human VPS29 protein (ab124582)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human VPS29 protein

    • Purity

      > 90 % SDS-PAGE.
      ab124582 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      Q9UBQ0

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSHMMLVLV LGDLHIPHRC NSLPAKFKKL LVPGKIQHIL CTGNLCTKES YDYLKTLAGD VHIVRGDFDE NLNYPEQKVV TVGQFKIGLI HGHQVIPWGD MASLALLQRQ FDVDILISGH THKFEAFEHE NKFYINPGSA TGAYNALETN IIPSFVLMDI QASTVVTYVY QLIGDDVKVE RIEYKKP

      • Predicted molecular weight

        23 kDa including tags

      • Amino acids

        1 to 182

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab124582 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 40% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • DC15
      • DC7
      • DKFZp564F0223
      • FLJ20492
      • hVPS29
      • PEP11
      • PEP11 homolog
      • Retromer protein
      • Vacuolar protein sorting 29
      • Vacuolar protein sorting 29 (yeast homolog)
      • Vacuolar protein sorting 29 (yeast)
      • Vacuolar protein sorting 29 homolog
      • Vacuolar protein sorting 29 homolog (S. cerevisiae)
      • Vacuolar protein sorting associated protein 29
      • Vacuolar protein sorting-associated protein 29
      • Vacuolar sorting protein VPS29/PEP11
      • Vesicle protein sorting 29
      • VPS 29
      • VPS29
      • VPS29 retromer complex component
      • VPS29_HUMAN
      • x 007 protein
      see all

    • Function

      Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro).

    • Tissue specificity

      Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain.

    • Sequence similarities

      Belongs to the VPS29 family.

    • Cellular localization

      Cytoplasm. Membrane. Endosome membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human VPS29 protein (ab124582)
      SDS-PAGE - Recombinant Human VPS29 protein (ab124582)
      15% SDS-PAGE showing ab124582 at approximately 23.2kDa (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab124582? Please let us know so that we can cite the reference in this datasheet.

    ab124582 has not yet been referenced specifically in any publications.

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