Recombinant human VPS34 protein (ab198071)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 70% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human VPS34 protein
See all VPS34 proteins and peptides -
Biological activity
Specific Activity: 140 pmol/min/μg.
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Purity
>= 70 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
GEAEKFHYIYSCDLDINVQLKIGSLEGKREQKSYKAVLEDPMLKFSGLYQ ETCSDLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLP RNAQVALTIWDVYGPGKAVPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEA DGSEPTKTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREI EMINESEKRSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFE LVKVPDPQMSMENLVESKHHKLARSLRSGPSDHDLKPNAATRDQLNIIVS YPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFLKCVNWDLPQEAKQALEL LGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQ ALKYENFDDIKNGLEPTKKDSQSSVSENVSNSGINSAEIDSSQIITSPLP SVSSPPPASKTKEVPDGENLEQDLCTFLISRACKNSTLANYLYWYVIVEC EDQDTQQRDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDR LVHLMKAVQRESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKI RGIIPETATLFKSALMPAQLFFKTEDGGKYPVIFKHGDDLRQDQLILQII SLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQ NFFRKYAPSENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLL TKTGKLFHIDFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEFRKQ CYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLS DEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK -
Predicted molecular weight
98 kDa including tags -
Amino acids
2 to 887 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
NM_002647.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
This product was previously labelled as PI 3 Kinase Class 3
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
80 µg/mL DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- hVps34
- MGC61518
- Phosphatidylinositol 3 kinase catalytic subunit type 3
see all -
Function
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. -
Tissue specificity
Ubiquitously expressed, with a highest expression in skeletal muscle. -
Sequence similarities
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. -
Cellular localization
Midbody. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab198071 has not yet been referenced specifically in any publications.