Overview

Description

  • Nature

    Recombinant
  • Source

    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      VRINGDGQE VLYLAEGDNV RLGCPYVLDP EDYGPNGLDI EWMQVNSDPA HHRENVFLSY QDKRINHGSL PHLQQRVRFA ASDPSQYDAS INLMNLQVSD TATYECRVKK TTMATRKVIV TVQARPAVPM CWTEGHMTYG NDVVLKCYAS GGSQPLSYKW AKISGHHYPY RAGSYTSQHS YHSELSYQES FHSSINQGLN NGDLVLKDIS RADDGLYQCT VANNVGYSVC VVEVKVSDSR RIG
    • Molecular weight

      29 kDa including tags
    • Amino acids

      22 to 263
    • Tags

      His tag C-Terminus
    • Additional sequence information

      (NP_001013683.1).

Specifications

Our Abpromise guarantee covers the use of ab220463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilized ab220463 at 10 μg/mL (100 μL/well) can bind Human B7-H5, Fc Tag with a linear range of 0.625-20 μg/mL.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level

    < 1.000 Eu/µg
  • Purity

    > 95 % SDS-PAGE.

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 95% PBS, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • C1orf204
    • Chromosome 1 open reading frame 204
    • EG240916
    • FLJ20442
    • FLJ39187
    • Hypothetical protein LOC2846772
    • RP11 190A12.5
    • V set and immunoglobulin domain containing 8
    • V-set and immunoglobulin domain-containing protein 8
    • VSIG8
    • VSIG8_HUMAN
    see all
  • Relevance

    Similarity: Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Images

  • SDS-PAGE analysis of reduced ab220463 stained overnight with Coomassie Blue.

    The protein migrates as 35-38 kDa under reducing condition (SDS-PAGE) due to glycosylation.

  • Immobilized ab220463 at 10 μg/mL (100 μL/well) can bind Human B7-H5, Fc Tag with a linear range of 0.625-20 μg/mL.

References

ab220463 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab220463.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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