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  1. Link

    recombinant-human-wdr5-protein-ab98079.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation
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Recombinant Human WDR5 protein (ab98079)

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SDS-PAGE - Recombinant Human WDR5 protein (ab98079)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

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    Description

    • Product name

      Recombinant Human WDR5 protein
      See all WDR5 proteins and peptides
    • Purity

      > 95 % SDS-PAGE.
      ab98079 was purified using conventional chromatography techniques.
    • Expression system

      Escherichia coli
    • Accession

      P61964
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGSSHHHHHHSSGLVPRGSHMATEEKKPETEAARAQPTPSSSATQSKPTP VKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFE KTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSN YVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFN RDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYIL AATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSED NLVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLW KSDC
      • Predicted molecular weight

        39 kDa including tags
      • Amino acids

        1 to 334
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-WDR5 antibody [2C2] (ab56919)

    Specifications

    Our Abpromise guarantee covers the use of ab98079 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Mass Spectrometry

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF
    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.0308% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    General Info

    • Alternative names

      • 2410008O07Rik
      • AA408785
      • AA960360
      • Big
      • BIG 3
      • BIG3
      • BMP2 induced 3 kb gene protein
      • BMP2 induced gene, 3-KB
      • BMP2-induced 3-kb gene protein
      • MGC114572
      • OTTHUMP00000162494
      • SWD 3
      • SWD3
      • SWD3 Set1c WD40 repeat protein homolog
      • WD repeat containing protein 5
      • WD repeat domain 5
      • WD repeat protein 5
      • WD repeat-containing protein 5
      • WD repeat-containing protein BIG-3
      • WDR 5
      • Wdr5
      • WDR5_HUMAN
      see all
    • Function

      Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.
    • Sequence similarities

      Belongs to the WD repeat WDR5/wds family.
      Contains 7 WD repeats.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P61964 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human WDR5 protein (ab98079)
      SDS-PAGE - Recombinant Human WDR5 protein (ab98079)
      15% SDS-PAGE analysis of 3µg ab98079

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab98079? Please let us know so that we can cite the reference in this datasheet.

    ab98079 has not yet been referenced specifically in any publications.

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