Key features and details
- Expression system: Escherichia coli
- Purity: > 75% SDS-PAGE
- Suitable for: WB, SDS-PAGE
Product nameRecombinant Human Wee1 protein
See all Wee1 proteins and peptides
Purity> 75 % SDS-PAGE.
Assessed by densitometry.
Expression systemEscherichia coli
Protein lengthProtein fragment
Predicted molecular weight115 kDa including tags
Amino acids247 to 646
Our Abpromise guarantee covers the use of ab125596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
- EC 188.8.131.52
FunctionActs as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated during M and G1 phases. Also autophosphorylated.
Ubiquitinated and degraded at the onset of G2/M phase.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125596 has not yet been referenced specifically in any publications.