Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 86% SDS-PAGE
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Human Wee1 protein (Tagged)
See all Wee1 proteins and peptides
Purity>= 86 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceMDTEKSGKRE FDVRQTPQVN INPFTPDSLL LHSSGQCRRR KRTYWNDSCG EDMEASDYEL EDETRPAKRI TITESNMKSR YTTEFHELEK IGSGEFGSVF KCVKRLDGCI YAIKRSKKPL AGSVDEQNAL REVYAHAVLG QHSHVVRYFS AWAEDDHMLI QNEYCNGGSL ADAISENYRI MSYFKEAELK DLLLQVGRGL RYIHSMSLVH MDIKPSNIFI SRTSIPNAAS EEGDEDDWAS NKVMFKIGDL GHVTRISSPQ VEEGDSRFLA NEVLQENYTH LPKADIFALA LTVVCAAGAE PLPRNGDQWH EIRQGRLPRI PQVLSQEFTE LLKVMIHPDP ERRPSAMALV KHSVLLSASR KSAEQLRIEL NAEKFKNSLL QKELKKAQMA KAAAEERALF TDRMATRSTT QSNRTSRLIG KKMNRSVSLT IY
Predicted molecular weight50 kDa including tags
Amino acids215 to 646
TagsDDDDK tag N-Terminus
Our Abpromise guarantee covers the use of ab271794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)
100 µg/mL DDDDK peptide
- EC 18.104.22.168
FunctionActs as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated during M and G1 phases. Also autophosphorylated.
Ubiquitinated and degraded at the onset of G2/M phase.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271794 has not yet been referenced specifically in any publications.