Recombinant human Wee1 protein (Tagged) (ab271795)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 75% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human Wee1 protein (Tagged)
See all Wee1 proteins and peptides -
Biological activity
Specific Activity: ≥42 pmol/min/μg
Assay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using Poly-(Lys4:Tyr) substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent.
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Purity
>= 75 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
DTEKSGKRE FDVRQTPQVN INPFTPDSLL LHSSGQCRRR KRTYWNDSCG EDMEASDYEL EDETRPAKRI TITESNMKSR YTTEFHELEK IGSGEFGSVF KCVKRLDGCI YAIKRSKKPL AGSVDEQNAL REVYAHAVLG QHSHVVRYFS AWAEDDHMLI QNEYCNGGSL ADAISENYRI MSYFKEAELK DLLLQVGRGL RYIHSMSLVH MDIKPSNIFI SRTSIPNAAS EEGDEDDWAS NKVMFKIGDL GHVTRISSPQ VEEGDSRFLA NEVLQENYTH LPKADIFALA LTVVCAAGAE PLPRNGDQWH EIRQGRLPRI PQVLSQEFTE LLKVMIHPDP ERRPSAMALV KHSVLLSASR KSAEQLRIEL NAEKFKNSLL QKELKKAQMA KAAAEERALF TDRMATRSTT QSNRTSRLIG KKMNRSVSLT IY -
Predicted molecular weight
75 kDa including tags -
Amino acids
215 to 646 -
Tags
GST tag N-Terminus -
Additional sequence information
N-terminal GST-Thrombin-Tag
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Specifications
Our Abpromise guarantee covers the use of ab271795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.06% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- DKFZp686I18166
- EC 2.7.10.2
- FLJ16446
see all -
Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated during M and G1 phases. Also autophosphorylated.
Ubiquitinated and degraded at the onset of G2/M phase. -
Cellular localization
Nucleus. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab271795 has not yet been referenced specifically in any publications.