Description

  • Product name

    Recombinant human WNK1 protein
    See all WNK1 proteins and peptides
  • Biological activity

    The Specific activity of ab105196 was determined to be 30 nmol/min/mg.
  • Purity

    > 95 % SDS-PAGE.
    Purity was determined to be >95% by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      67 kDa including tags
    • Amino acids

      181 to 507

Specifications

Our Abpromise guarantee covers the use of ab105196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Erythrocyte 65 kDa protein
    • HSAN2
    • HSN2
    • hWNK1
    • KDP
    • KIAA0344
    • Kinase deficient protein
    • MGC163339
    • MGC163341
    • p65
    • PPP1R167
    • PRKWNK1
    • Prostate derived sterile 20 like kinase
    • Protein kinase lysine deficient 1
    • Protein kinase lysine-deficient 1
    • Protein kinase with no lysine 1
    • Protein phosphatase 1, regulatory subunit 167
    • PSK
    • Serine/threonine protein kinase WNK1
    • Serine/threonine protein kinase WNK1 1
    • Serine/threonine protein kinase WNK1 2
    • Serine/threonine-protein kinase WNK1
    • With no K
    • WNK lysine deficient protein kinase 1
    • WNK lysine deficient protein kinase 1 isoform
    • WNK1
    • WNK1_HUMAN
    see all
  • Function

    Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
  • Tissue specificity

    Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
  • Involvement in disease

    Pseudohypoaldosteronism 2C
    Neuropathy, hereditary sensory and autonomic, 2A
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    O-glycosylated.
    Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • The Specific activity of ab105196 was determined to be 30 nmol/min/mg.
  • SDS-PAGE showing ab105196 at approximately 67kDa.

References

ab105196 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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