The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
Protein Wnt 5a
Wingless type MMTV integration site family member 5A
WNT 5A protein
WNT 5A protein precursor
WNT5A protein precursor
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level).
Belongs to the Wnt family.
Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor binding. Glycosylation is necessary for secretion but not for activity.
Secreted > extracellular space > extracellular matrix.
SDS-PAGE - Recombinant Human Wnt5a protein (ab159811)
ab159811 on a 12.5% SDS-PAGE stained with Coomassie Blue.
This product has been referenced in:
Bai D et al. Attenuation of Focal Adhesion Kinase Reduces Lipopolysaccharide-Induced Inflammation Injury through Inactivation of the Wnt and NF-?B Pathways in A549 Cells. Biochemistry (Mosc)82:446-453 (2017).
Read more (PubMed: 28371601) »