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Cell Biology Apoptosis Intracellular Survivin / IAPs
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Recombinant human XIAP protein (ab95933)

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Functional Studies - Recombinant human XIAP protein (ab95933)
  • SDS-PAGE - Recombinant human XIAP protein (ab95933)
  • Western blot - Recombinant human XIAP protein (ab95933)

Key features and details

  • Expression system: Baculovirus
  • Active: Yes
  • Tags: DDDDK tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE, WB

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Description

  • Product name

    Recombinant human XIAP protein
    See all XIAP proteins and peptides
  • Biological activity

    Specific Activity: 0.0015 pmol/min/µg Assay conditions: 4 mM ATP, 5mM MgCl2 (both required for reaction), 1mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotinylated Ubiquitin (1 µg) in 50mM Tris pH 7.4.
  • Purity

    65 % .
    Purity is >65% by SDS-PAGE.
  • Expression system

    Baculovirus
  • Accession

    P98170
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      57 kDa including tags
    • Amino acids

      2 to 497
    • Tags

      DDDDK tag N-Terminus

Associated products

  • Related Products

    • Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
    • Anti-XIAP antibody (ab21278)
    • Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
    • Anti-XIAP antibody (ab2541)

Specifications

Our Abpromise guarantee covers the use of ab95933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C.

    pH: 8.00
    Constituents: 0.0462% DTT, 0.395% Tris HCl, 0.05% Tween, 20% Glycerol, 0.58% Sodium chloride

    104 µg/ml FLAG peptide

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • AP 13
    • API3
    • Apoptosis Inhibitor 3
    • Baculoviral IAP repeat containing 4
    • Baculoviral IAP Repeat Containing Protein 4
    • Baculoviral IAP repeat-containing protein 4
    • BIRC 4
    • BIRC4
    • E3 ubiquitin-protein ligase XIAP
    • hIAP-3
    • hIAP3
    • HILP
    • IAP 3
    • IAP like protein
    • IAP-3
    • IAP-like protein
    • IAP3
    • ILP
    • ILP 1
    • ILP1
    • Inhibitor of apoptosis protein 3
    • Inhibitor of Apoptosis X Linked
    • Mammalian IAP Homologue A
    • MIHA
    • X linked IAP
    • X linked inhibitor of apoptosis
    • X linked inhibitor of apoptosis E3 ubiquitin protein ligase
    • X linked inhibitor of apoptosis protein
    • X-linked IAP
    • x-linked inhibitor of apopotosis proteins
    • X-linked inhibitor of apoptosis protein
    • Xiap
    • XIAP_HUMAN
    • XLP2
    see all
  • Function

    Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
  • Tissue specificity

    Ubiquitous, except peripheral blood leukocytes.
  • Involvement in disease

    Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
  • Sequence similarities

    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 RING-type zinc finger.
  • Domain

    The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
  • Post-translational
    modifications

    Ubiquitinated and degraded by the proteasome in apoptotic cells.
    Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P98170 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human XIAP protein (ab95933)
    Functional Studies - Recombinant human XIAP protein (ab95933)
    Image showing specific activity of ab95933.
  • SDS-PAGE - Recombinant human XIAP protein (ab95933)
    SDS-PAGE - Recombinant human XIAP protein (ab95933)
    SDS-PAGE showing ab95933 at approximately 57kDa (5µg).
  • Western blot - Recombinant human XIAP protein (ab95933)
    Western blot - Recombinant human XIAP protein (ab95933)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab95933? Please let us know so that we can cite the reference in this datasheet.

    ab95933 has not yet been referenced specifically in any publications.

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