Recombinant human YKL-40/CHI3L1 protein (ab182706)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human YKL-40/CHI3L1 protein
See all YKL-40/CHI3L1 proteins and peptides -
Biological activity
Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. When 50000 cells per well are added to rhCHI3-L1 coated plate, cell adhesion is enhanced in a dose dependent manner.
The ED50 for this effect is typically 0.8 – 3.5 µg/ml. -
Purity
> 95 % SDS-PAGE.
Purified by Immobilized metal affinity chromatography. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEW NDVTLYGMLN TLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTF IKSVPPFLRTHGFDGLDLAWLY PGRRDKQHFTTLIKEMKAEFIKEAQP GKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFI SIMTYDFHGAWRGT TGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTF GR SFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILG QQVPYATK GNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQG SFCGQDLRFPLTNAIKDALA AT -
Predicted molecular weight
41 kDa including tags -
Amino acids
22 to 383 -
Tags
His tag C-Terminus -
Additional sequence information
Mature protein. NP_001267.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab182706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Additional notes
Previously labelled as CHI3L1.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
General Info
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Alternative names
- 39 kDa synovial protein
- ASRT7
- Cartilage glycoprotein 39
see all -
Function
Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. -
Tissue specificity
Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts. -
Involvement in disease
A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7) [MIM:611960]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. -
Sequence similarities
Belongs to the glycosyl hydrolase 18 family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab182706 has been referenced in 2 publications.
- Xue Q et al. Downregulation of Interleukin-13 Receptor α2 Inhibits Angiogenic Formation Mediated by Chitinase 3-Like 1 in Late Atherosclerotic Lesions of apoE-/- Mice. Front Physiol 12:690109 (2021). PubMed: 34349665
- Chen J et al. Early detection of Alzheimer's disease by peptides from phage display screening. Brain Res 1721:146306 (2019). PubMed: 31247207