Recombinant Human YOD1 protein (ab140732)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE, MS

Description

  • Product name

    Recombinant Human YOD1 protein
    See all YOD1 proteins and peptides

  • Purity

    > 90 % SDS-PAGE.
    ab140732 was purified using conventional chromatography techniques.

  • Expression system

    Escherichia coli

  • Accession

    Q5VVQ6

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMGSMFGPAKGRHFGVHPAPGFPGGVSQQAA GTKAGPAGAWPVGSRTDTMWRLRCKAKDGTHVLQGLSSRTRVRELQGQIA AITGIAPGGQRILVGYPPECLDLSNGDTILEDLPIQSGDMLIIEEDQTRP RSSPAFTKRGASSYVRETLPVLTRTVVPADNSCLFTSVYYVVEGGVLNPA CAPEMRRLIAQIVASDPDFYSEAILGKTNQEYCDWIKRDDTWGGAIEISI LSKFYQCEICVVDTQTVRIDRFGEDAGYTKRVLLIYDGIHYDPLQRNFPD PDTPPLTIFSSNDDIVLVQALELADEARRRRQFTDVNRFTLRCMVCQKGL TGQAEAREHAKETGHTNFGEV

    • Predicted molecular weight

      41 kDa including tags

    • Amino acids

      1 to 348

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab140732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

General Info

  • Alternative names

    • DUBA-8
    • DUBA8
    • HIN-7
    • HIV-1-induced protease 7
    • HsHIN7
    • OTU domain containing 2
    • OTU domain-containing protein 2
    • OTU1_HUMAN
    • OTUD2
    • PRO0907
    • RP11-164O23.1
    • Ubiquitin thioesterase OTU1
    • yod1
    • YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)
    • YOD1 OTU deubiquinating enzyme 1 homolog (yeast)
    • YOD1 OTU deubiquinating enzyme 1, S. cerevisiae, homolog of
    see all

  • Function

    Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Able to cleave both polyubiquitin and di-ubiquitin.

  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 OTU domain.

  • Domain

    The UBAX-like region mediates the interaction with VCP. According to PubMed:19818707, it corresponds to a UBX domain, which is a hallmark for VCP-associated proteins. However, no canonical UBX is detected by prediction tools such as Pfam or PROSITE.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human YOD1 protein (ab140732)
    SDS-PAGE - Recombinant Human YOD1 protein (ab140732)
    15% SDS-PAGE analysis of ab140732 (3µg).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab140732? Please let us know so that we can cite the reference in this datasheet.

ab140732 has not yet been referenced specifically in any publications.

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