Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human ZCH11 protein (Tagged)
See all ZCH11 proteins and peptides
Purity>= 90 % SDS-PAGE.
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceSQKQQTCTDN TGDSDDSASG IEDVSDDLSK MKNDESNKEN SSEMDYLENA TVIDESALTP EQRLGLKQAE ERLERDHIFR LEKRSPEYTN CRYLCKLCLI HIENIQGAHK HIKEKRHKKN ILEKQEESEL RSLPPPSPAH LAALSVAVIE LAKEHGITDD DLRVRQEIVE EMSKVITTFL PECSLRLYGS SLTRFALKSS DVNIDIKFPP KMNHPDLLIK VLGILKKNVL YVDVESDFHA KVPVVVCRDR KSGLLCRVSA GNDMACLTTD LLTALGKIEP VFIPLVLAFR YWAKLCYIDS QTDGGIPSYC FALMVMFFLQ QRKPPLLPCL LGSWIEGFDP KRMDDFQLKG IVEEKFVKWE CNSSSATEKN SIAEENKAKA DQPKDDTKKT ETDNQSNAMK EKHGKSPLAL ETPNRVSLGQ LWLELLKFYT LDFALEEYVI CVRIQDILTR ENKNWPKRRI AIEDPFSVKR NVARSLNSQL VYEYVVERFR AAYRYFACPQ TKGGNKSTVD FKKREKGKIS NKKPVKSNNM ATNGCILLGE TTEKINAERE QPVQCDEMDC TSQRCIIDNN NLLVNELDFA DHGQDSSSLS TSKSSEIEPK LDKKQDDLAP SETCLKKELS QCNCIDLSKS PDPDKSTGTD CRSNLETESS HQSVCTDTSA TSCNCKATED ASDLNDDDNL PTQELYYVFD KFILTSGKPP TIVCSICKKD GHSKNDCPED FRKIDLKPLP PMTNRFREIL DLVCKRCFDE LSPPCSEQHN REQILIGLEK FIQKEYDEKA RLCLFGSSKN GFGFRDSDLD ICMTLEGHEN AEKLNCKEII ENLAKILKRH PGLRNILPIT TAKVPIVKFE HRRSGLEGDI SLYNTLAQHN TRMLATYAAI DPRVQYLGYT MKVFAKRCDI GDASRGSLSS YAYILMVLYF LQQRKPPVIP VLQEIFDGKQ IPQRMVDGWN AFFFDKTEEL KKRLPSLGKN TESLGELWLG LLRFYTEEFD FKEYVISIRQ KKLLTTFEKQ WTSKCIAIED PFDLNHNLGA GVSRKMTNFI MKAFINGRKL FGTPFYPLIG REAEYFFDSR VLTDGELAPN DRCCRVCGKI GHYMKDCPKR KS
Predicted molecular weight127 kDa including tags
Amino acids212 to 1313
TagsDDDDK tag C-Terminus
Our Abpromise guarantee covers the use of ab271776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
100 ug/mL DDDDK peptide
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- EC 22.214.171.124
FunctionUridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation.
Sequence similaritiesBelongs to the DNA polymerase type-B-like family.
Contains 3 CCHC-type zinc fingers.
Contains 2 PAP-associated domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271776 has not yet been referenced specifically in any publications.