Recombinant Human ZP2 protein (ab159886)

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SDS-PAGE - Recombinant Human ZP2 protein (ab159886)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: WB, ELISA

    Description

    • Product name

      Recombinant Human ZP2 protein

    • Expression system

      Wheat germ

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MACRQRGGSWSPSGWFNAGWSTYRSISLFFALVTSGNSIDVSQLVNPAFP GTVTCDEREITVEFPSSPGTKKWHASVVDPLGLDMPNCTYILDPEKLTLR ATYDNCTRRVHGGHQMTIRVMNNSAALRHGAVMYQFFCPAMQVEETQGLS ASTICQKDFMSFSLPRVFSGLADDSKGTKVQMGWSIEVGDGARAKTLTLP EAMKEGFSLLIDNHRMTFHVPFNATGVTHYVQGNSHLYMVSLKLTFISPG QKVIFSSQAICAPDPVTCNATHMTLTIPEFPGKLKSVSFENQNIDVSQLH DNGIDLEATNGMKLHFSKTLLKTKLSEKCLLHQFYLASLKLTFLLRPETV SMVIYPECLCESPVSIVTGELCTQDGFMDVEVYSYQTQPALDLGTLRVGN SSCQPVFEAQSQGLVRFHIPLNGCGTRYKFEDDKVVYENEIHALWTDFPP SKISRDSEFRNDMLLNINVESLTPPVASVKLGPFTLILQSYPDNSYQQPY GENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQW NVVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSS LVYFHCSALICNRLSPDSPLCSVTCPVSSRHRRATGATEAEKMTVSLPGP ILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGAMDTKGHKTA GDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH

      • Amino acids

        1 to 736

      • Tags

        GST tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab159886 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      ELISA

    • Form

      Liquid

    • Additional notes

       

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • OTTHUMP00000115849
      • Processed zona pellucida sperm-binding protein 2
      • Zona pellucida glycoprotein 2
      • zona pellucida glycoprotein ZP2
      • Zona pellucida protein A
      • Zp-2
      • ZP2
      • ZP2_HUMAN
      • ZPA
      see all

    • Function

      The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.

    • Tissue specificity

      Oocytes.

    • Sequence similarities

      Belongs to the ZP domain family. ZPA subfamily.
      Contains 1 ZP domain.

    • Developmental stage

      Expressed during the 2-week growth phase of oogenesis, prior to ovulation.

    • Domain

      The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

    • Post-translational
      modifications

      Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
      Proteolytically cleaved in the N-terminal part after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy.
      O-glycosylated; contains sulfate-substituted glycans.

    • Cellular localization

      Secreted > extracellular space > extracellular matrix and Cell membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human ZP2 protein (ab159886)
      SDS-PAGE - Recombinant Human ZP2 protein (ab159886)
      ab159886 on a 12.5% SDS-PAGE stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab159886? Please let us know so that we can cite the reference in this datasheet.

    ab159886 has not yet been referenced specifically in any publications.

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