Recombinant Human ZP2 protein (ab159886)

Product name

Recombinant Human ZP2 protein
See all ZP2 proteins and peptides
Protein length

Full length protein

Nature

Recombinant
Source

Wheat germ
Amino Acid Sequence
- Species
  
  Human
- Sequence
  
  MACRQRGGSWSPSGWFNAGWSTYRSISLFFALVTSGNSIDVSQLVNPAFP GTVTCDEREITVEFPSSPGTKKWHASVVDPLGLDMPNCTYILDPEKLTLR ATYDNCTRRVHGGHQMTIRVMNNSAALRHGAVMYQFFCPAMQVEETQGLS ASTICQKDFMSFSLPRVFSGLADDSKGTKVQMGWSIEVGDGARAKTLTLP EAMKEGFSLLIDNHRMTFHVPFNATGVTHYVQGNSHLYMVSLKLTFISPG QKVIFSSQAICAPDPVTCNATHMTLTIPEFPGKLKSVSFENQNIDVSQLH DNGIDLEATNGMKLHFSKTLLKTKLSEKCLLHQFYLASLKLTFLLRPETV SMVIYPECLCESPVSIVTGELCTQDGFMDVEVYSYQTQPALDLGTLRVGN SSCQPVFEAQSQGLVRFHIPLNGCGTRYKFEDDKVVYENEIHALWTDFPP SKISRDSEFRNDMLLNINVESLTPPVASVKLGPFTLILQSYPDNSYQQPY GENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQW NVVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSS LVYFHCSALICNRLSPDSPLCSVTCPVSSRHRRATGATEAEKMTVSLPGP ILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGAMDTKGHKTA GDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH
- Amino acids
  
  1 to 736
- Tags
  
  proprietary tag N-Terminus

Our Abpromise guarantee covers the use of ab159886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

Western blot

ELISA
Form

Liquid
Additional notes

Protein concentration is above or equal to 0.05 mg/ml.
Concentration information loading...

Stability and Storage

Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl

Alternative names
- OTTHUMP00000115849
- Processed zona pellucida sperm-binding protein 2
- Zona pellucida glycoprotein 2
- zona pellucida glycoprotein ZP2
- Zona pellucida protein A
- Zp-2
- ZP2
- ZP2_HUMAN
- ZPA
see all
Function

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.
Tissue specificity

Oocytes.
Sequence similarities

Belongs to the ZP domain family. ZPA subfamily.
Contains 1 ZP domain.
Developmental stage

Expressed during the 2-week growth phase of oogenesis, prior to ovulation.
Domain

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
Post-translational
modifications

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
Proteolytically cleaved in the N-terminal part after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy.
O-glycosylated; contains sulfate-substituted glycans.
Cellular localization

Secreted > extracellular space > extracellular matrix and Cell membrane.
Target information above from: UniProt accession Q05996 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

SDS-PAGE - Recombinant Human ZP2 protein (ab159886)

ab159886 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Datasheet

ab159886 has not yet been referenced specifically in any publications.

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Recombinant Human ZP2 protein (ab159886)

Overview

Description

Specifications

Preparation and Storage

General Info

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References

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