Recombinant Human ZP2 protein (ab159886)

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Description

  • Product name

    Recombinant Human ZP2 protein

  • Expression system

    Wheat germ

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MACRQRGGSWSPSGWFNAGWSTYRSISLFFALVTSGNSIDVSQLVNPAFP GTVTCDEREITVEFPSSPGTKKWHASVVDPLGLDMPNCTYILDPEKLTLR ATYDNCTRRVHGGHQMTIRVMNNSAALRHGAVMYQFFCPAMQVEETQGLS ASTICQKDFMSFSLPRVFSGLADDSKGTKVQMGWSIEVGDGARAKTLTLP EAMKEGFSLLIDNHRMTFHVPFNATGVTHYVQGNSHLYMVSLKLTFISPG QKVIFSSQAICAPDPVTCNATHMTLTIPEFPGKLKSVSFENQNIDVSQLH DNGIDLEATNGMKLHFSKTLLKTKLSEKCLLHQFYLASLKLTFLLRPETV SMVIYPECLCESPVSIVTGELCTQDGFMDVEVYSYQTQPALDLGTLRVGN SSCQPVFEAQSQGLVRFHIPLNGCGTRYKFEDDKVVYENEIHALWTDFPP SKISRDSEFRNDMLLNINVESLTPPVASVKLGPFTLILQSYPDNSYQQPY GENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQW NVVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSS LVYFHCSALICNRLSPDSPLCSVTCPVSSRHRRATGATEAEKMTVSLPGP ILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGAMDTKGHKTA GDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH

    • Amino acids

      1 to 736

    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab159886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form

    Liquid

  • Additional notes

     

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • OTTHUMP00000115849
    • Processed zona pellucida sperm-binding protein 2
    • Zona pellucida glycoprotein 2
    • zona pellucida glycoprotein ZP2
    • Zona pellucida protein A
    • Zp-2
    • ZP2
    • ZP2_HUMAN
    • ZPA
    see all

  • Function

    The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.

  • Tissue specificity

    Oocytes.

  • Sequence similarities

    Belongs to the ZP domain family. ZPA subfamily.
    Contains 1 ZP domain.

  • Developmental stage

    Expressed during the 2-week growth phase of oogenesis, prior to ovulation.

  • Domain

    The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

  • Post-translational
    modifications

    Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
    Proteolytically cleaved in the N-terminal part after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy.
    O-glycosylated; contains sulfate-substituted glycans.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix and Cell membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human ZP2 protein (ab159886)
    SDS-PAGE - Recombinant Human ZP2 protein (ab159886)
    ab159886 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab159886 has not yet been referenced specifically in any publications.

Publishing research using ab159886? Please let us know so that we can cite the reference in this datasheet.

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