Recombinant human/mouse Ihh protein (Animal Free) (ab205517)
Key features and details
- Expression system: Escherichia coli
- Purity: > 97% SDS-PAGE
- Endotoxin level: < 0.050 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human/mouse Ihh protein (Animal Free)
See all Ihh proteins and peptides -
Biological activity
The activity is determined by the dose-dependent induction of alkaline phosphatase acitivity of CCL-226 cells and is typically less than 10 µg/mL. This corresponds to an expected specific activity of 1 x 102 units/mg.
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Purity
> 97 % SDS-PAGE.
Purity is typically 97% as determined by reducing and non-reducing SDS-PAGE. -
Endotoxin level
< 0.050 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
Yes -
Nature
Recombinant -
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Species
Mouse -
Sequence
MIIGPGRVVG SRRRPPRKLV PLAYKQFSPN VPEKTLGASG RYEGKIARSS ERFKELTPNY NPDIIFKDEE NTGADRLMTQ RCKDRLNSLA ISVMNQWPGV KLRVTEGWDE DGHHSEESLH YEGRAVDITT SDRDRNKYGL LARLAVEAGF DWVYYESKAH VHCSVKSEHS AAAKTGG -
Predicted molecular weight
20 kDa -
Amino acids
29 to 202
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Description
Recombinant mouse Ihh protein (Animal Free)
Specifications
Our Abpromise guarantee covers the use of ab205517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Additional notes
This product shares 100% homology with amino acids 29 -202 of Human IHH.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 0.1% Trifluoroacetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge vial before opening. Suspend the product by gently pipetting 250 ul of 10mM HCl down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
General Info
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Alternative names
- BDA1
- HHG-2
- HHG2
see all -
Function
Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). -
Tissue specificity
Expressed in embryonic lung, and in adult kidney and liver. -
Involvement in disease
Defects in IHH are the cause of brachydactyly type A1 (BDA1) [MIM:112500]. BDA1 is an autosomal dominant disorder characterized by middle phalanges of all the digits rudimentary or fused with the terminal phalanges. The proximal phalanges of the thumbs and big toes are short.
Defects in IHH are a cause of acrocapitofemoral dysplasia (ACFD) [MIM:607778]. ACFD is a disorder characterized by short stature of variable severity with postnatal onset. The most constant radiographic abnormalities are observed in the tubular bones of the hands and in the proximal part of the femur. Cone-shaped epiphyses or a similar epiphyseal configuration with premature epimetaphyseal fusion result in shortening of the skeletal components involved. Cone-shaped epiphyses were also present to a variable extent at the shoulders, knees, and ankles. -
Sequence similarities
Belongs to the hedgehog family. -
Post-translational
modificationsThe C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
Palmitoylated. N-palmitoylation is required for N-product multimerization and full activity. -
Cellular localization
Secreted > extracellular space. The C-terminal peptide diffuses from the cell and Cell membrane. The N-terminal peptide remains associated with the cell surface. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab205517 has not yet been referenced specifically in any publications.