Recombinant human/mouse/rat Activin A protein (ab151687)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant human/mouse/rat Activin A protein
See all Activin A proteins and peptides -
Biological activity
The activity is determined by the ability to induce cytoxicity of MPC-11 cells and is typically less than 2 ng/mL. Corresponding to a specific activity of 5 x 105 units/mg.
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Purity
>= 95 % SDS-PAGE.
Purity is typically >95% as determined by HPLC, Reducing and Non-reducing SDS-PAGE. -
Endotoxin level
<=1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS -
Predicted molecular weight
26 kDa -
Amino acids
311 to 426 -
Additional sequence information
Non-glycosylated homodimer, containing two 117 amino acid chains. Activin A is a homodimer of two beta A chains and is not biologically active until the N terminal propeptide is cleaved from each.
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Description
Recombinant human Activin A protein
Specifications
Our Abpromise guarantee covers the use of ab151687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
HPLC
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Form
Lyophilised -
Additional notes
This product shares 100% homology with amino acids 309 - 424 of Rat and Mouse Activin A.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C.
Constituent: 0.02% Trifluoroacetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water, which can then be further diluted to other aqueous solutions. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
General Info
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Alternative names
- Activin A
- Activin beta A chain
- Activin beta-A chain
see all -
Function
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human/mouse/rat Activin A protein (ab151687)Functional analysis of ab151687 -
SDS-PAGE - Recombinant human/mouse/rat Activin A protein (ab151687)SDS PAGE analysis of ab151687 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
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Functional Studies - Recombinant human/mouse/rat Activin A protein (ab151687)Induction of cytotoxicity in MPC-11 cells using ab151687. Assays were carried out in duplicate.
Datasheets and documents
References
ab151687 has not yet been referenced specifically in any publications.
