Recombinant Human/Murine/Rat BDNF protein (Active) (ab9794)

I heard back from both labs that produce the BDNF and pro-BDNF proteins, and both said that the reconstitution instructions should be followed directly.
BDNF ab9794: reconstitute in water
pro BDNF ab138787: reconstitute in PBS

Thank you for contacting us.
The lab let me know the following:
The protein is lyophilized with no additives, which means that it is HPLC purified. Therefore, the only buffer that would be present at lyophilization would be water and 0.1% TFA (Triflouroacetic acid). This buffer is volatile, and will therefore evaporate out during the lyophilization process. Therefore, only a trace amount of this buffer would be present in the final formulation of the lyophilized protein.
The protein is > 98% pure by HPLC and SDS-PAGE.
Thus, we are not sure what is in the protein solution that could kill the birds, unless trace amounts of TFA could have such devastating effect.
We do however have not tested the protein in this way and also do not recommend using our products for in vivo studies.
I hope this information is of some help to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

Vorweg: Den Rabattcode können Sie ab sofort nutzen. Da der vorige unseren Unterlagen zufolge gerade abgelaufen ist, habe ich Ihnen nochmal einen neuen ausgestellt: x.

Ihre Protokolldetails, die wir zusätzlich für unsere Qualitätskontrolle verwenden, bestätigen meine bisherige Vermutung, dass das Problem auf das Protein ab9794 zurückzuführen ist und Sie offenbar ein defektes Röhrchen erhalten haben. Wir möchten uns dafür entschuldigen, und da ich weiß, dass Sie viel Zeit für Ihre Experimente verwendet haben, möchte Ihnen einen kostenlosen Ersatz (miteinem neuen Vial ab9794 oder einem Protein Ihrer Wahl)odereine Gutschrift als Ersatz anbieten. Welche Alternative würden Sie bevorzugen?

Um dies in die Wege zu leiten, benötige ich die ursprüngliche Bestellnummer. Können Sie mir die Bestellnummern x vom ddmmyy bestätigen?

Vielen Dank für Ihre Kooperation und Hilfsbereitschaft. Ich freue mich bald wieder von Ihnen zu hören.

Vielen Dank für Ihre Anfrage.

Es tut mir leid zu hören, dass Sie Probleme mit unserem BDNF-Antikörper und Proteinhaben.

Bezüglich des Gutscheincodes kann ich Ihnen mitteilen, dass ich die Ausnahme machen konnte, den Rabattcode zu aktivieren. Normalerweise sollte die Abbildung über unser Abreviewsystem eingereicht werden, damit der Codevon unserem System erkannt werden kann. Da Sie uns aber die Ergebnisse dankenswerterweise per email geschickt hatten und wirden Fall daher nochgenauer nachverfolgen können, reicht das indiesem Fall aus. Sie können mit demCode also ab sofort ein kostenloses Protein von uns erhalten (www.abcam.com/collaborationdiscount).

Vielen Dankfür Ihre Abbildung: In der Tat scheint es ein Problem mit entweder demProtein oder dem Antikörper zu geben. Um dieszu ergründen, benötigen wir noch ein paar genauere Angaben zu Ihrem Protokoll und Ihren Proben (wie haben Sie zum Beispiel das rekombinante Protein vor Auftragen ins Gel behandelt? Was genau ist M in der Abbildung; der Marker?). Ich habe daher unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen erhalten wir alle nötigen Informationen, die auch für unsere Qualitätskontrolle wichtig sind.

Sobald Sie dieses Formular an uns zurückgeschickt haben, werden wir uns Ihr Protokoll ansehen und möglichst Veränderungsvorschläge machen, die Ihre Ergebnisse verbessern werden. Falls sich herausstellt, dass der Antikörper und/oder das Protein nicht so funktionieren, wie auf dem Datenblatt beschrieben, werden wir Ihnen gerne einen Ersatz oder eine Gutschrift schicken.

Ich freue mich, bald wieder von Ihnen zu hören.

Thank you for your phone call. I am sending you the testing discount codes for the 2 proteins for trying them in a blocking experiment - see below.

Please send me your WB data so that I cantake a look at your results.
DISCOUNT CODE: xxx for ab9794
DISCOUNT CODE: xxx for ab113151
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab9794 and ab113151in blocking. Each code will give you 1 freePROTEIN before the expiration date. To redeem this offer, please submit one Abreview each for blockingand include each code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Thank you for contacting us. The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions: HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRID TSCV CTLTIKRGR which corresponds to the residues 129 - 247, and is based on the protein database entry http://www.uniprot.org/uniprot/P23560 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

DISCOUNT CODE:xxxx Expiration date: dd.mm.2012 I am very pleased to hear you would like to use ab9794 and send us an image of your results. This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an Abreview with the image for Western blot and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you very much for your call. Firstly, I would like to mention that we also have a PC12 cell lysate available that could be used as positive control for ab108383, as stated on the datasheet. I just point this out as a lysate may be of use as a control not only for this BDNF antibody, but for other antibodies in future experiments as well. This would be for example ab14883: Click here (or use the following: https://www.abcam.com/PC12-cytoplasmic-extract-lysate-ab14883.html). However, if you prefer the protein as positive control as discussed on the phone, it is absolutely fine to use ab9794 (Click here (or use the following: https://www.abcam.com/Recombinant-HumanMurineRat-BDNF-protein-Active-ab9794.html).) As mentioned this morning, we do not currently have an image of ab9794 in SDS-PAGE/Western blot. Therefore, I can offer a discount off a future purchase if you buyab9794 now, use it in SDS-PAGE/Western blot and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free protein. It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and send an image of ab9794 in SDS-PAGE/Western blot. I will then send a discount code. This code must be issued before purchasing ab9794 so please wait for my reply before ordering. 2. Purchase ab9794 either by phone, fax, or online (www.abcam.com). 3. Use it in SDS-PAGE/Western blot. 4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any protein ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Furthermore, we are always happy to offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. So if you need any advice or help with ab108383, please feel free to contact us. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount

Thank you for your enquiry and patience. Our source for this protein has told me that yes, the protein can be reconstituted to a concentration of 1 mg/ml with water. If you have any additional questions, please contact us again.