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  1. Link

    recombinant-humanmurinerat-bdnf-protein-active-ab9794.pdf

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Bioactive grade

Recombinant Human/Murine/Rat BDNF protein (Active) (ab9794)

  • Datasheet
  • SDS
Submit a review Q&A (9)References (17)

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Western blot - Recombinant Human/Murine/Rat BDNF protein (ab9794)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 98% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Suitable for: HPLC, Cellular Activation, SDS-PAGE, WB

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    Description

    • Product name

      Recombinant Human/Murine/Rat BDNF protein (Active)
      See all BDNF proteins and peptides
    • Biological activity

      Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.

      The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions.

    • Purity

      > 98 % SDS-PAGE.
      > 98% pure HPLC analyses.
    • Endotoxin level

      < 1.000 Eu/µg
    • Expression system

      Escherichia coli
    • Accession

      P23560
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Sequence

        MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
      • Predicted molecular weight

        27 kDa
      • Amino acids

        129 to 247
      • Additional sequence information

        aa 129 to 247 refers to the full length mature form. This product does not contain signal peptide or propeptide.

    Specifications

    Our Abpromise guarantee covers the use of ab9794 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      HPLC

      Cellular Activation

      SDS-PAGE

      Western blot

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.

    General Info

    • Alternative names

      • Abrineurin
      • ANON2
      • BDNF
      • BDNF_HUMAN
      • Brain Derived Neurotrophic Factor
      • Brain-derived neurotrophic factor
      • BULN2
      • MGC34632
      • Neurotrophin
      see all
    • Function

      During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
    • Tissue specificity

      Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
    • Involvement in disease

      Bulimia nervosa 2
      Congenital central hypoventilation syndrome
    • Sequence similarities

      Belongs to the NGF-beta family.
    • Post-translational
      modifications

      The propeptide is N-glycosylated and glycosulfated.
      Converted into mature BDNF by plasmin (PLG).
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P23560 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Western blot - Recombinant Human/Murine/Rat BDNF protein (ab9794)
      Western blot - Recombinant Human/Murine/Rat BDNF protein (ab9794)
      Rabbit Anti-BDNF antibody at 1 µg/ml + Recombinant Human/Murine/Rat BDNF protein (Active) (ab9794) at 0.1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 4 minutes

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (17)

    Publishing research using ab9794? Please let us know so that we can cite the reference in this datasheet.

    ab9794 has been referenced in 17 publications.

    • Kim HJ  et al. Brain-Derived Neurotrophic Factor Secreting Human Mesenchymal Stem Cells Improve Outcomes in Rett Syndrome Mouse Models. Front Neurosci 15:725398 (2021). PubMed: 34690674
    • Kalinichenko SG  et al. Brain-Derived Neurotrophic Factor (BDNF) As a Regulator of Apoptosis under Conditions of Focal Experimental Stroke. Bull Exp Biol Med 169:701-706 (2020). PubMed: 32990850
    • Klimovich P  et al. Three-Dimensional Model of Dorsal Root Ganglion Explant as a Method of Studying Neurotrophic Factors in Regenerative Medicine. Biomedicines 8:N/A (2020). PubMed: 32138155
    • Huang S  et al. Prenatal Dexamethasone Exposure Induced Alterations in Neurobehavior and Hippocampal Glutamatergic System Balance in Female Rat Offspring. Toxicol Sci N/A:N/A (2019). PubMed: 31518422
    • Wang Y  et al. Dexmedetomidine attenuates the toxicity of ß-amyloid on neurons and astrocytes by increasing BDNF production under the regulation of HDAC2 and HDAC5. Mol Med Rep 19:533-540 (2019). PubMed: 30483749
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-9 of 9 Abreviews or Q&A

    Question

    Customer kindly contacted us regarding reconstitution of ab9794 (BDNF) and ab138787 (proBDNF).

    Read More

    Abcam community

    Verified customer

    Asked on Dec 14 2012

    Answer


    I heard back from both labs that produce the BDNF and pro-BDNF proteins, and both said that the reconstitution instructions should be followed directly.
    BDNF ab9794: reconstitute in water
    pro BDNF ab138787: reconstitute in PBS

    Read More

    Abcam Scientific Support

    Answered on Dec 14 2012

    Question

    protein 96% pure
    uses protein to infuse into birds (30 g sparrows) and they die due to this (untested application)
    What are the other 4% impurities?
    What else is in the lyophlized powder (buffer composition before hand)?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 26 2012

    Answer

    Thank you for contacting us.
    The lab let me know the following:
    The protein is lyophilized with no additives, which means that it is HPLC purified. Therefore, the only buffer that would be present at lyophilization would be water and 0.1% TFA (Triflouroacetic acid). This buffer is volatile, and will therefore evaporate out during the lyophilization process. Therefore, only a trace amount of this buffer would be present in the final formulation of the lyophilized protein.
    The protein is > 98% pure by HPLC and SDS-PAGE.
    Thus, we are not sure what is in the protein solution that could kill the birds, unless trace amounts of TFA could have such devastating effect.
    We do however have not tested the protein in this way and also do not recommend using our products for in vivo studies.
    I hope this information is of some help to you. Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 26 2012

    Question

    Sehr geehrte Damen und Herren,
    anliegen möchte ich mich nochmals an Sie wenden, um Ihnen den beantworteten Fragebogen zum Thema WB beantwortet zurück zuschicken. Sie finden außerdem in Anhang die Abbildung des Western Blots mit dem Rekombinanten Protein als Nachweis, dass wir keine BDNF nachweisen konnten.
    Bitte geben Sie mir Rückmeldung, wenn ich den o.g. Rabatt Code einlösen kann, um einen neuen Antikörper bei Ihrer Firma bestellen zu können.
    Mit freundlichen Grüßen

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2012

    Answer

    Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

    Vorweg: Den Rabattcode können Sie ab sofort nutzen. Da der vorige unseren Unterlagen zufolge gerade abgelaufen ist, habe ich Ihnen nochmal einen neuen ausgestellt: x.

    Ihre Protokolldetails, die wir zusätzlich für unsere Qualitätskontrolle verwenden, bestätigen meine bisherige Vermutung, dass das Problem auf das Protein ab9794 zurückzuführen ist und Sie offenbar ein defektes Röhrchen erhalten haben. Wir möchten uns dafür entschuldigen, und da ich weiß, dass Sie viel Zeit für Ihre Experimente verwendet haben, möchte Ihnen einen kostenlosen Ersatz (miteinem neuen Vial ab9794 oder einem Protein Ihrer Wahl)odereine Gutschrift als Ersatz anbieten. Welche Alternative würden Sie bevorzugen?

    Um dies in die Wege zu leiten, benötige ich die ursprüngliche Bestellnummer. Können Sie mir die Bestellnummern x vom ddmmyy bestätigen?

    Vielen Dank für Ihre Kooperation und Hilfsbereitschaft. Ich freue mich bald wieder von Ihnen zu hören.

    Read More

    Abcam Scientific Support

    Answered on Mar 29 2012

    Question

    Sehr geehrte Damen und Herren,habe ein Anliegen bzgl. eines bei Ihnen bestellten Antikörpers, den wir mit einem speziellen rekombinanten Protein ebenfalls von Ihrer Firma ausgetestet hatten. Wir haben den AK getestet mit einer AK Verdünnung von 1:200 und hatten leider nie ein Bande nachweisen können.
    Dann haben wir bei Ihrer Firma das rekombinante Protein BDNF bestellt, sozusagen als positive Kontrolle für den Antikörper, und auch da konnten wir mit dem BDNF AK keine Banden sehen. (siehe Anlage)
    Ein wissenschaftlicher Mitarbeiter unserer Arbeitsgruppe, hatte bereits mit Ihnen Kontakt aufgenommen und ihm wurde nach Einreichen der entsprechenden Western Blots Bildes eine Gutschrift bei einer nächsten Bestellung versprochen.
    Der BDNF Antikörper hat die Best. Nr. ab108383 und das BDNF rekombinanten Protein die Bestell. Nr. ab9794.
    Über eine Antwort und den Erhalt eines Gutschein Codes würde ich mich sehr freuen.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 21 2012

    Answer

    Vielen Dank für Ihre Anfrage.

    Es tut mir leid zu hören, dass Sie Probleme mit unserem BDNF-Antikörper und Proteinhaben.

    Bezüglich des Gutscheincodes kann ich Ihnen mitteilen, dass ich die Ausnahme machen konnte, den Rabattcode zu aktivieren. Normalerweise sollte die Abbildung über unser Abreviewsystem eingereicht werden, damit der Codevon unserem System erkannt werden kann. Da Sie uns aber die Ergebnisse dankenswerterweise per email geschickt hatten und wirden Fall daher nochgenauer nachverfolgen können, reicht das indiesem Fall aus. Sie können mit demCode also ab sofort ein kostenloses Protein von uns erhalten (www.abcam.com/collaborationdiscount).

    Vielen Dankfür Ihre Abbildung: In der Tat scheint es ein Problem mit entweder demProtein oder dem Antikörper zu geben. Um dieszu ergründen, benötigen wir noch ein paar genauere Angaben zu Ihrem Protokoll und Ihren Proben (wie haben Sie zum Beispiel das rekombinante Protein vor Auftragen ins Gel behandelt? Was genau ist M in der Abbildung; der Marker?). Ich habe daher unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen erhalten wir alle nötigen Informationen, die auch für unsere Qualitätskontrolle wichtig sind.

    Sobald Sie dieses Formular an uns zurückgeschickt haben, werden wir uns Ihr Protokoll ansehen und möglichst Veränderungsvorschläge machen, die Ihre Ergebnisse verbessern werden. Falls sich herausstellt, dass der Antikörper und/oder das Protein nicht so funktionieren, wie auf dem Datenblatt beschrieben, werden wir Ihnen gerne einen Ersatz oder eine Gutschrift schicken.

    Ich freue mich, bald wieder von Ihnen zu hören.

    Read More

    Abcam Scientific Support

    Answered on Mar 21 2012

    Question

    WB with ab10505
    sees multiple bands (isoforms, mature form)
    human cell line samples
    will send results
    send discount code for proteins ab9794 and ab113151- to be used in blocking experiment

    Read More

    Abcam community

    Verified customer

    Asked on Feb 20 2012

    Answer

    Thank you for your phone call. I am sending you the testing discount codes for the 2 proteins for trying them in a blocking experiment - see below.

    Please send me your WB data so that I cantake a look at your results.
    DISCOUNT CODE: xxx for ab9794
    DISCOUNT CODE: xxx for ab113151
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab9794 and ab113151in blocking. Each code will give you 1 freePROTEIN before the expiration date. To redeem this offer, please submit one Abreview each for blockingand include each code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Feb 20 2012

    Question

    send protein sequence the protein represents which isoform/variant ?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 22 2011

    Answer

    Thank you for contacting us. The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions: HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRID TSCV CTLTIKRGR which corresponds to the residues 129 - 247, and is based on the protein database entry http://www.uniprot.org/uniprot/P23560 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Dec 22 2011

    Question

    We would like to proceed and purchase the ab9794. When we will do the Western I will send you an image of the gel.  I will wait for your email with the discount code.  

    Read More

    Abcam community

    Verified customer

    Asked on Nov 24 2011

    Answer

    DISCOUNT CODE:xxxx Expiration date: dd.mm.2012 I am very pleased to hear you would like to use ab9794 and send us an image of your results. This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an Abreview with the image for Western blot and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Nov 24 2011

    Question

    We are looking for a positive control. Can you recommend one?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 22 2011

    Answer

    Thank you very much for your call. Firstly, I would like to mention that we also have a PC12 cell lysate available that could be used as positive control for ab108383, as stated on the datasheet. I just point this out as a lysate may be of use as a control not only for this BDNF antibody, but for other antibodies in future experiments as well. This would be for example ab14883: Click here (or use the following: https://www.abcam.com/PC12-cytoplasmic-extract-lysate-ab14883.html). However, if you prefer the protein as positive control as discussed on the phone, it is absolutely fine to use ab9794 (Click here (or use the following: https://www.abcam.com/Recombinant-HumanMurineRat-BDNF-protein-Active-ab9794.html).) As mentioned this morning, we do not currently have an image of ab9794 in SDS-PAGE/Western blot. Therefore, I can offer a discount off a future purchase if you buyab9794 now, use it in SDS-PAGE/Western blot and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free protein. It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and send an image of ab9794 in SDS-PAGE/Western blot. I will then send a discount code. This code must be issued before purchasing ab9794 so please wait for my reply before ordering. 2. Purchase ab9794 either by phone, fax, or online (www.abcam.com). 3. Use it in SDS-PAGE/Western blot. 4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any protein ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Furthermore, we are always happy to offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. So if you need any advice or help with ab108383, please feel free to contact us. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount

    Read More

    Abcam Scientific Support

    Answered on Nov 22 2011

    Question

    Is it possible to reconstitute this protein to a concentration of 1 mg/ml? Customer needs to know this for her particular application.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 07 2005

    Answer

    Thank you for your enquiry and patience. Our source for this protein has told me that yes, the protein can be reconstituted to a concentration of 1 mg/ml with water. If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Jun 15 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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