Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant IL-1RA protein (Animal Free)
See all IL-1RA proteins and peptides
Inhibition of human IL-1 alpha induced proliferation of D10.G4.1 cells.
ED50 ≤ 50 ng/ml (≥ 2.0 x 104 units/mg).
Purity>= 95 % SDS-PAGE.
Purity determined by reducing and non-reducing SDS-PAGE.
Endotoxin level<=1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMRPSGRKSSKMQAFRIWDVNQKTFYLRNNQLVAGYLQGPNVNLEEKIDVV PIEPHALFLGIHGGKMCLSCVKSGDETRLQLEAVNITDLSENRKQDKRFA FIRSDSGPTTSFESAACPGWFLCTAMEADQPVSLTNMPDEGVMVTKFYFQ EDE
Predicted molecular weight17 kDa
Amino acids26 to 177
Additional sequence informationThis product is the mature full length protein from aa 26 to 177. The signal peptide is not included.
Our Abpromise guarantee covers the use of ab229599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: 0.16% Sodium phosphate
Lyophilized from a sterile (0.2 micron) filtered aqueous solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile water at 0.1 mg/ml. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
- ICIL 1RA
FunctionInhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Tissue specificityThe intracellular form of IL1RN is predominantly expressed in epithelial cells.
Involvement in diseaseMicrovascular complications of diabetes 4
Interleukin 1 receptor antagonist deficiency
Cellular localizationCytoplasm and Secreted.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229599 has not yet been referenced specifically in any publications.