Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant Influenza A Virus Hemagglutinin H7 protein
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Predicted molecular weight62 kDa
Additional sequence informationFrom virus strain A/Anhui/1/2013 (H7N9).
Our Abpromise guarantee covers the use of ab190421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Recombinant hemagglutinin glycoprotein provides a control protein for immunoassays and a tool to enhance Orthomyxovirus research.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituent: 99% Tris buffered saline
Contains 0.01% non-ionic detergent.
- Hemagglutinin 7
RelevanceHaemagglutinin (HA) is one of two main surface fusion glycoproteins embedded in the envelope of influenza viruses, the other being neuraminidase (NA). There are sixteen known HA subtypes (H1-H16) and nine NA subtypes (N1-N9), which together are used to classify influenza viruses (e.g. H5N1). The antigenic variations in HA and NA enable the virus to evade host antibodies made to previous influenza strains, accounting for recurrent influenza epidemics.
Hemagglutination with turkey red blood cells: Hemagglutinin Titer 1:2048
SDS-PAGE analysis of 0.4 µg ab190421 under reducing conditions.
The theoretical molecular weight of the protein is ~62 kDa, without glycosylation. Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms.
Anti-H7 NY mouse antiserum at 1/100 dilution +
Recombinant Influenza A Virus Hemagglutinin H7 protein (ab190421) at 0.38 µg
Anti-mouse AP conjugate
Quality control testing demonstrates strong detection of GP null under reduced conditions when using anti-H7 influenza antiserum.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab190421 has been referenced in 1 publication.
- Novoseltseva AA et al. Structural and Functional Aspects of G-Quadruplex Aptamers Which Bind a Broad Range of Influenza A Viruses. Biomolecules 10:N/A (2020). PubMed: 31936820