Recombinant Japanese encephalitis virus NS1 glycoprotein (His tag) (ab218555)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Description
-
Product name
Recombinant Japanese encephalitis virus NS1 glycoprotein (His tag) -
Purity
> 95 % SDS-PAGE.
ab218555 was 0.2 um filter sterilised. -
Expression system
HEK 293 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Japanese encephalitis virus -
Sequence
DTGCAIDITRKEMRCGSGIFVHNDVEAWVDRYKYLPETPRSLAKIVHKAH KEGVCGVRSVTRLEHQMWEAVRDELNVLLKENAVDLSVVVNKPVGRYRSA PKRLSMTQEKFEMGWKAWGKSILFAPELANSTFVVDGPETKECPDEHRAW NSMQIEDFGFGITSTRVWLKIREESTDECDGAIIGTAVKGHVAVHSDLSY WIESRYNDTWKLERAVFGEVKSCTWPETHTLWGDDVEESELIIPHTIAGP KSKHNRREGYKTQNQGPWDENGIVLDFDYCPGTKVTITEDCSKRGPSVRT TTDSGKLITDWCCRSCSLPPLRFRTENGCWYGMEIRPVMHDETTLVRSQV DA -
Predicted molecular weight
40 kDa -
Amino acids
795 to 1146 -
Tags
His tag C-Terminus -
Additional sequence information
SA-14 strain. ab218555 is presented in 2 distinct oligomeric states with molecular weights of 335 kDa and 450 kDa, as determined by size exclusion chromatography,
-
-
Description
Recombinant Japanese encephalitis virus Japanese encephalitis virus NS1 glycoprotein (His tag)
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab218555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Form
Liquid -
Additional notes
The antigen is in its native folding state, and possesses all post-translational modifications. Unlike other flavivirus NS1 proteins which secrete as a single hexameric form, Japanese Encephalitis virus NS1 shows two oligomeric states in approximately equal proportion.
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7
Constituent: 100% PBS
(Dulbecco’s PBS).
General Info
-
Alternative names
- Japanese encephalitis non-structural protein 1
- Japanese encephalitis NS1
- Japanese encephalitis virus non-structural protein 1
-
Relevance
The Japanese encephalitis viral genome encodes 7 non-structural proteins NS1-NS5. NS1 contains N-linked carbohydrate chains at positions 130 and 207. It is not incorporated into the virion but exists in the host cell, on the cell surface and can also be extracellular.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab218555 has not yet been referenced specifically in any publications.