• Nature

  • Source

    Saccharomyces cerevisiae
  • Amino Acid Sequence


Our Abpromise guarantee covers the use of ab74569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications


    Western blot


  • Purity

    > 95 % SDS-PAGE.
    Purified by ultracentifugation.
  • Form

  • Additional notes

    JCV VP1 protein was purified and lyophilized assembled into virus like particles (VLPs). It is tested for hemagglutination activity and analysed using electron microscopy. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    Constituent: PBS

  • Reconstitution
    Reconstitute with deionized H2O. After reconstitution store at 4°C.

General Info

  • Alternative names

    • Capsid protein VP1
  • Relevance

    The human polyomavirus JC virus (JCV) infects greater than 80% of the human population. The JC virus is a small (38-40 nm in diameter) double stranded, circular DNA virus covered by an icosahedral capsid. Infection with JCV is asymptomatic and it occurs in early childhood. After the primary infection, the virus remains in latent state in the kidney, until it's reactivation under immunosuppressive conditions to result in Progressive Multifocal Leukoencephalopathy (PML), a fatal demyelinating disease. 70% of all HIV-1- infected patients will exhibit neurological disorders and between 5 and 8% of all HIV-1-infected patients will develop PML. Similar to other polyomaviruses, JCV can cause tumors when intracerebrally inoculated at high titers into developing rodent. Several reports suggest the association of viruses, especially of the polyomavirus family with different types of human brain tumors. Tumorigenecity of JCV is most likely induced by the viral early gene product T-antigen. T-antigen has the capacity to interact with several tumor suppressor proteins, most notably p53, and functionally inactivate these proteins.
  • Cellular localization

    Virion. Nucleus


  • SDS-PAGE showing ab74569 (4µg/lane).
    Lane 1 reprents the molecular weight ladder. From the bottom: 14.4, 18.4, 25.0, 35.0, 45.0, 66.2 kDa


This product has been referenced in:

  • López-Moncada F  et al. Nucleolar Expression and Chromosomal Associations in Robertsonian Spermatocytes of Mus musculus domesticus. Genes (Basel) 10:N/A (2019). Read more (PubMed: 30736350) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A


In regards to the JCV VP1 Protein, ab74569. JCV Polyomavirus Major Capsid VP1 protein is supplied in the form of VLP (Virus-like particles). Actually it is a higher structure formed from pentamers, but not pentamers itself.

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Thank you for contacting us.

JCV VP1 was purified assembled into virus like particles (VLPs).

VLP is the structure formed from pentamers, but in fact it is not pentamer or monomer form of VP1.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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We have been informed by the lab that JCV VP1 was purified and lyophilized assembled into virus like particles (VLPs). VLP structure is formed from pentamers, but in fact it is not pentamer or monomer form of VP1.

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Die Spezifitätsnotiz beschreibt folgendes:

ab34756 bindet das Major Capsidprotein VP1 von humanem Polyoma Virus JCV und das Capsidprotein VP1 von hamster polyomavirus (HaPyV) and Simian Virus 40 (SV40).

ab34756 kreuz-reagiert nicht mit dem Major Capsidprotein VP1 von humanem polyomavirus BKV (anderer Stamm) und murinem polyomavirus (MuPyV).

Ich musste es auch dreimal lesen :-)

D.H., dass ab34756 mit ab74569 (JCV Polyomavirus Major Capsid VP1 protein) als Positivkontrolle verwendet werden kann.

Bitte beachten Sie, dass ab34756 ein Mausantikörper ist und keine direkten Vergleiche mit humanem IgG oder IgM gezogen werden können. Als Test ob der ELISA grundsätzlich funktioniert ist er aber geeignet.

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Thank you for your reply. I can confirm that the antibody ab34756 will react with the SV40 VP1 protein ab74565. On the antibody datasheet in the section labelled "cross-reactivity", it notes that it does react with the VP1 protein of SV40. I hope this will give you some reassurance. Please let me know if you have any additional questions and I'll be happy to help.

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Thank you for your call this week and for your patience while I have been in touch with the production lab about these proteins. I have confirmed with the lab that these proteins do not contain any tag. To detect them, you could use specific monoclonal antibody ab34756, which reacts with both proteins- https://www.abcam.com/Human-Polyoma-virus-JCV-capsid-protein-VP1-antibody-ab34756.html I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

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