Recombinant listeria monocytogenes Listeriolysin (LLO) protein (ab68198)

Description

  • Product name

    Recombinant listeria monocytogenes Listeriolysin (LLO) protein
    See all Listeriolysin (LLO) proteins and peptides
  • Biological activity

    Activity: ab68198 has a hemolytic activity 1.25x106 HU/mg. 2mM DTT is used to reactivate the toxin.
  • Purity

    > 90 % SDS-PAGE.
    Purity: Greater than 90.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Listeria monocytogenes
    • Amino acids

      26 to 529

Specifications

Our Abpromise guarantee covers the use of ab68198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Additional notes


    This protein can be used for: Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituents: 0.6% Monobasic dihydrogen sodium phosphate, 0.02025% Potassium chloride, 0.82% Sodium phosphate, 0.0154% DTT, 0.0292% EDTA, 5% Glycerol, 2.9% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • hly
    • hlyA
    • lisA
    • LLO
    • Thiol activated cytolysin
    see all
  • Relevance

    Listeria monocyogenes is a faculative intracellular Gram-positive food borne bacterium, increasingly recognized as being responsible for severe infections in both humans and animals. Ingestion of contaminated food causes an infection called Listeriosis, which affects especially immunocomprimised patients, new borns and pregnant women. It is characterized by severe syndromes such as encephalitis, meningoencephalitis, septicemia and abortion. Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol. LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.

References

ab68198 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

The unit of activity mentioned on the datasheet of ab68198, Listeriolysin full length protein, HU/mg (hemolytic units) is defined in the attached paper,

High-level expression of the Listeria monocytogenes listeriolysin O in Escherichia coli and preliminary characterization of the purified protein. Protein Expr Purif. 2003 Mar;28(1):78-85. PMID: 12651110.

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