Key features and details
- Expression system: Escherichia coli
- Purity: > 90% Ion Exchange Chromatography
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant listeria monocytogenes Listeriolysin (LLO) protein
Hemolytic activity: 1.25x106 HU/mg. 2 mM DTT could be use to reactivate the toxin.
Purity> 90 % Ion Exchange Chromatography.
Purified by hydroxyapatite and ion-exchange chromatography
Expression systemEscherichia coli
Protein lengthProtein fragment
Amino acids60 to 529
Our Abpromise guarantee covers the use of ab83345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.02025% Potassium chloride, 0.82% Sodium phosphate, 0.0154% DTT, 0.0292% EDTA, 5% Glycerol, 2.9% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
RelevanceListeria monocyogenes is a faculative intracellular Gram-positive food borne bacterium, increasingly recognized as being responsible for severe infections in both humans and animals. Ingestion of contaminated food causes an infection called Listeriosis, which affects especially immunocomprimised patients, new borns and pregnant women. It is characterized by severe syndromes such as encephalitis, meningoencephalitis, septicemia and abortion. Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol. LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab83345 has been referenced in 1 publication.
- Coelho C et al. Listeria monocytogenes virulence factors, including listeriolysin O, are secreted in biologically active extracellular vesicles. J Biol Chem 294:1202-1217 (2019). PubMed: 30504226