Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Suitable for: WB, ELISA, SDS-PAGE, Functional Studies
Product nameRecombinant LukD protein
Cytotoxicity can be detected in Human neutrophils when used in combination with LukE in a concentration range of 12.5-800 nM.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMKIEKLGKSSVASSIALLLLSNTVDAAQNITPKREKKVDDKITLYKTTAT SDNDKLNIFQILTFNFIKDKSYDKDTLVLKAAGNINSGYKNSNPKDYNYS QFYWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINI SNGLSGGLNGSKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNN GWGPYGRDSYDPTYGNELFLGGDKSSSNAGQNFLPTHQIPLLARGNFNPE FISVLSHKLFDTKKSKIKVTYQREMDRYTNQWNRSHWVGNNYKNQNTVTF TSTYEVDWQNILLKLIGTDSKETNPGV
Predicted molecular weight37 kDa
Amino acids1 to 327
Additional sequence informationFrom Staphylococcus aureus. Purified.
Our Abpromise guarantee covers the use of ab190423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituent: 100% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Leukotoxin LukD
SDS-PAGE analysis of ab190423: 1 µg (lane 1) and 5 µg (lane 2).
Anti-LukF-PV polyclonal antibody at 0.5 µg/ml +
Recombinant LukD protein (ab190423) at 0.1 µg
Anti-rabbit IgG-HRP conjugate
Toxin Functionality: Human promyelocytic leukemia cell line HL60 was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of rLukE and ab190423 alone (Open circles and open squares, respectively) or equimolar concentration of both proteins (Closed circles) for 3 hours at 37°C with 5% CO2 and 95% humidity. Cellular viability was determined by adding XTT and incubation for additional 16 hours. Cells were centrifuged and the OD determined in the supernatants at 470nm. EC50 was found to be 95.1 nM of each toxin subunit.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab190423 has not yet been referenced specifically in any publications.