Recombinant mouse AMPK alpha 1 + AMPK gamma 1 + AMPK beta 2/PRKAB2 protein (Active) (ab268344)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant mouse AMPK alpha 1 + AMPK gamma 1 + AMPK beta 2/PRKAB2 protein (Active) -
Biological activity
The specific activity of ab268344 was 340 nmol/min/min in a peptide kinase assay using SAMStide (HMRSAMSGLHLVKRR) as substrate.
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Purity
> 75 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Mouse -
Sequence
MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTG HKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDI FMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRD LKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRL YAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNP SVISLLKHMLQVDPMKRAAIKDIREHEWFKQDLPKYLFPEDPSYSSTMID DEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKD FYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQ GVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNP VTSTFSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSISNY RSCQRSDSDAEAQGKPSDVSLTSSVTSLDSSPVDVAPRPGSHTIEFFEMC ANLIKILAQ -
Molecular weight information
SDS-PAGE molecular weight: ~68-72 kDa -
Amino acids
1 to 559 -
Tags
His tag N-Terminus -
Additional sequence information
GeneBank: NM_001013367
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Amino Acid Sequence 2
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Species
Mouse -
Sequence
MGNTTSERVSGERHGAKAARAEGGGHGPGKEHKIMVGSTDDPSVFSLPDS KLPGDKEFVPWQQDLDDSVKPAQQARPTVIRWSEGGKEVFISGSFNNWST KIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINN LIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYVFRSEER FKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVM VLSATHRYKKKYVTTLLYKPI -
Molecular weight information
SDS-PAGE molecular weight: ~35 kDa -
Amino acids
1 to 271 -
Tags
His tag N-Terminus -
Additional sequence information
GeneBank: NM_182997
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Amino Acid Sequence 3
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Species
Mouse -
Sequence
MESVAAESSPALENEHFQETPESNNSVYTSFMKSHRCYDLIPTSSKLVVF DTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKS ALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIH RLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLQELQIGTY ANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAA EKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLV VVDEHDVVKGIVSLSDILQALVLTGGEKKP -
Molecular weight information
SDS-PAGE molecular weight: ~40 kDa -
Amino acids
1 to 330 -
Tags
His tag N-Terminus -
Additional sequence information
GeneBank: NM_016781
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab268344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.82% Sodium phosphate, 1.74% Sodium chloride, 0.002% PMSF, 0.004% DTT, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab268344 has not yet been referenced specifically in any publications.