Description

  • Product name

    Recombinant Mouse B2M + FCGRT protein
    See all B2M + FCGRT proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Amino Acid Sequence 1

    • Species

      Mouse
    • Sequence

      SETRPPLMYHLTAVSNPSTGLPSFWATGWLGPQQYLTYNSLRQEADPCGA WMWENQVSWYWEKETTDLKSKEQLFLEALKTLEKILNGTYTLQGLLGCEL ASDNSSVPTAVFALNGEEFMKFNPRIGNWTGEWPETEIVANLWMKQPDAA RKESEFLLNSCPERLLGHLERGRRNLEWKEPPSMRLKARPGNSGSSVLTC AAFSFYPPELKFRFLRNGLASGSGNCSTGPNGDGSFHAWSLLEVKRGDEH HYQCQVEHEGLAQPLTVDLDSSARSS
    • Predicted molecular weight

      33 kDa including tags
    • Amino acids

      22 to 297
    • Tags

      His tag C-Terminus
    • Additional sequence information

      Extracellular domain of FCGRT.
    • Amino Acid Sequence 2

    • Species

      Mouse
    • Sequence

      IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVE MSDMSFSKDWSFYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDM
    • Predicted molecular weight

      15 kDa including tags
    • Amino acids

      21 to 119
    • Tags

      proprietary tag C-Terminus
    • Additional sequence information

      This product is the mature full length B2M protein from aa21 - 119. The signal peptide is not included. B2M is fused with Twin-Strep tag at the C-terminus.

Specifications

Our Abpromise guarantee covers the use of ab207141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    ab207141 is a mouse FCGRT and beta 2 Microglobulin heterodimer protein, produced by co-expression of FCGRT and B2M.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    pH: 7.40
    Constituents: 95% PBS, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing.

General Info

  • Alternative names

    • beta chain of MHC class I molecules
    • beta-2-microglobin
    • Beta-2-microglobulin
    • c fragment of IgG, receptor, transporter, alpha
    • Fc Fragment of IgG, receptor transporter, alpha
    • FcRn
    • FcRn alpha chain
    • IgG Fc fragment receptor transporter alpha chain
    • IgG receptor FcRn large subunit p51
    • immunoglobulin receptor, intestinal, heavy chain
    • major histocompatibility complex class I-like Fc receptor
    • Neonatal Fc receptor
    • neonatal Fc-receptor for Ig
    see all
  • Relevance

    FCGRT + B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT has a possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
  • Cellular localization

    FCGRT: Cell membrane; Single-pass type I membrane protein B2M: Secreted

Images

  • DTT-reduced SDS-PAGE analysis of ab207141 stained overnight with Coomassie Blue.
    DTT-reduced protein migrates as 45-50 kDa (FCGRT) and 18 kDa (B2M) respectively in SDS-PAGE due to glycosylation.

References

ab207141 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab207141.
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