Recombinant mouse BAFF-R protein (Fc Chimera) (ab157042)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Mouse
    • Sequence
      GARRLRVRSQRSRDSSVPTQCNQTECFDPLVRNCVSCELFHTPDTGHTSS LEPGTALQPQ EGSALRPDV
    • Molecular weight
      35 kDa
    • Amino acids
      2 to 70
    • Additional sequence information
      The extracellular domain of mouse BAFF Receptor (aa 2-70) is fused at the C-terminus to a linker peptide (10 aa) and the Fc portion of human IgG1.

Specifications

Our Abpromise guarantee covers the use of ab157042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Blocks the binding of mouse and Human BAFF to their BAFF-receptors, inhibiting BAFF-mediated splenocyte survival.
  • Applications

    Functional Studies

    SDS-PAGE

    Flow Cytometry

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    >95% by SDS-PAGE .

  • Form
    Lyophilised
  • Additional notes
    Binds Mouse and Human BAFF. Blocks the binding of mouse and Human BAFF to their BAFF-receptors, inhibiting BAFF-mediated splenocyte survival.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C.

    Constituent: 99% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

General Info

  • Alternative names
    • B cell activating factor receptor
    • B-cell-activating factor receptor
    • BAFF R
    • BAFF receptor
    • BAFF-R
    • BAFFR
    • BLyS receptor 3
    • BlySR3
    • BR 3
    • BR3
    • BROMIX
    • CD 268
    • CD268
    • CD268 antigen
    • CVID4
    • MGC138235
    • OTTHUMP00000028746
    • Prolixin
    • TNFRSF 13C
    • Tnfrsf13c
    • TR13C_HUMAN
    • Tumor necrosis factor receptor subunit member 13C
    • Tumor necrosis factor receptor superfamily member 13C
    see all
  • Function
    B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response.
  • Tissue specificity
    Highly expressed in spleen and lymph node, and in resting B-cells. Detected at lower levels in activated B-cells, resting CD4+ T-cells, in thymus and peripheral blood leukocytes.
  • Involvement in disease
    Defects in TNFRSF13C are the cause of immunodeficiency common variable type 4 (CVID4) [MIM:613494]; also called antibody deficiency due to BAFFR defect. CVID4 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities
    Contains 1 TNFR-Cys repeat.
  • Cellular localization
    Membrane.
  • Information by UniProt

Images

  • Inhibition of BAFF-mediated splenocyte survival. ab157042 inhibits BAFF-mediated activation of freshly isolated splenocytes. Concentration of ab157042 to achieve complete inhibition depends on the concentration of soluble BAFF used. A ratio (soluble BAFF:ab157042) 1:50 and higher is recommended. Method: On day 0 splenocytes were isolated from a freshly collected C57Bl6 spleen. An aliquot of the splenocytes was analyzed on FACS, and B cells were gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media alone, with the indicated concentration of recombinant Human soluble BAFF alone or in the presence of an excess of ab157042 (50µg/ml). After three days in culture, cells were again analyzed on FACS with the saved setting. Splenocytes survival index (=ratio of %living/%dead cells) was calculated and plotted.

References

ab157042 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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