Bioactive grade

Recombinant mouse C10 protein (Active) (ab243257)

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SDS-PAGE - Recombinant mouse C10 protein (Active) (ab243257)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 97% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Suitable for: SDS-PAGE, Functional Studies, HPLC

    Description

    • Product name

      Recombinant mouse C10 protein (Active)
      See all C10 proteins and peptides

    • Biological activity

      Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells is in a concentration range of 10-100 ng/ml.

    • Purity

      > 97 % SDS-PAGE.
      > 97 % by HPLC

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      Escherichia coli

    • Accession

      P27784

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Mouse

      • Sequence

        GLIQEIEKEDRRYNPPIIHQGFQDTSSDCCFSYATQIPCKRFIYYFPTSG GCIKPGIIFISRRGTQVCADPSDRRVQRCLSTLKQGPRSGNKVIA

      • Predicted molecular weight

        11 kDa

      • Amino acids

        22 to 116

      • Additional sequence information

        Full-length mature chain lacking the signal peptide.

    Specifications

    Our Abpromise guarantee covers the use of ab243257 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

      HPLC

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituent: PBS

      0.2 µm filtered

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Briefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 °C or below. Further dilutions should be made in appropriate buffered solutions.

    General Info

    • Alternative names

      • C10
      • CCL 6
      • chemokine (C-C motif) ligand 6
      • Chemokine C C motif Ligand 6
      • MRP1
      • SCYA6
      • Small Inducible Cytokine A6
      see all

    • Relevance

      CCL6 (C10) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. It is a potent chemoattractant of macrophages, but it can also attract B cells, CD4+ lymphocytes and eosinophils. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be induced under conditions suitable for myeloid cell differentiation. CCL6 (C10) was originally identified as a transcript that is induced in bone marrow cells upon stimulation of GMCSF. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

    • Cellular localization

      Secreted

    Images

    • SDS-PAGE - Recombinant mouse C10 protein (Active) (ab243257)
      SDS-PAGE - Recombinant mouse C10 protein (Active) (ab243257)
      SDS Page analysis of ab243257

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab243257? Please let us know so that we can cite the reference in this datasheet.

    ab243257 has not yet been referenced specifically in any publications.

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