Question (69057) | Recombinant mouse Caspase-1 protein (ab52079)

Go to datasheet (ab52079)

Question

reconstituted in 100 ul PBS (per DS instructions)
used 1 or 2 ug protein + 1 or 4 units caspase 1 in assay buffer for 1h at 37 degree

sees no cleavage
SDS-PAGE: protein substrate = 30 kDa, sees 30kDa band before and after cleavage
would expect to see 10 and 20 kD band

run enzyme alone on SDS-PAGE: sees also other higher bands

Answer

Send free of charge replacement with another lot.

Sign up