Recombinant Mouse CD13 protein (His tag) (ab276741)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 97% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Mouse CD13 protein (His tag)
See all CD13 proteins and peptides -
Purity
> 97 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
DESKPWNQYR LPKTLIPDSY RVILRPYLTP NNQGLYIFQG NSTVRFTCNQ TTDVIIIHSK KLNYTLKGNH RVVLRTLDGT PAPNIDKTEL VERTEYLVVH LQGSLVEGRQ YEMDSQFQGE LADDLAGFYR SEYMEGDVKK VVATTQMQAA DARKSFPCFD EPAMKAMFNI TLIYPNNLIA LSNMLPKESK PYPEDPSCTM TEFHSTPKMS TYLLAYIVSE FKNISSVSAN GVQIGIWARP SAIDEGQGDY ALNVTGPILN FFAQHYNTSY PLPKSDQIAL PDFNAGAMEN WGLVTYRESS LVFDSQSSSI SNKERVVTVI AHELAHQWFG NLVTVAWWND LWLNEGFASY VEYLGADYAE PTWNLKDLMV LNDVYRVMAV DALASSHPLS SPADEIKTPD QIMELFDSIT YSKGASVIRM LSSFLTEDLF KKGLSSYLHT YQYSNTVYLD LWEHLQKAVN QQTAVQPPAT VRTIMDRWIL QMGFPVITVN TNTGEISQKH FLLDSKSNVT RPSEFNYIWI APIPFLKSGQ EDHYWLDVEK NQSAKFQTSS NEWILLNINV TGYYLVNYDE NNWKKLQNQL QTDLSVIPVI NRAQIIHDSF NLASAKMIPI TLALDNTLFL VKEAEYMPWQ AALSSLNYFT LMFDRSEVYG PMKRYLKKQV TPLFFYFQNR TNNWVNRPPT LMEQYNEINA ISTACSSGLK ECRDLVVELY SQWMKNPNNN TIHPNLRSTV YCNAIAFGGE EEWNFAWEQF RNATLVNEAD KLRSALACSK DVWILNRYLS YTLNPDYIRK QDTTSTIISI ASNVAGHPLV WDFVRSNWKK LFENYGGGSF SFANLIQGVT RRFSSEFELQ QLEQFKADNS ATGFGTGTRA LEQALEKTRA NIDWVKENKD AVFKWFTENS S -
Predicted molecular weight
104 kDa including tags -
Molecular weight information
The recombinant mouse ANPEP consists of 909 amino acids and has a calculated molecular mass of 104 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 120-130 kDa protein in SDS-PAGE under reducing conditions. -
Amino acids
66 to 966 -
Tags
His tag C-Terminus -
Additional sequence information
Predicted N-terminus: Lys 66.
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Specifications
Our Abpromise guarantee covers the use of ab276741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 100% PBS -
ReconstitutionThis information is lot specific. Please contact our technical Support team for details.
General Info
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Alternative names
- Alanyl (membrane) aminopeptidase
- Alanyl aminopeptidase
- Aminopeptidase M
see all -
Function
Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. -
Tissue specificity
Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients. -
Sequence similarities
Belongs to the peptidase M1 family. -
Domain
Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies). -
Post-translational
modificationsSulfated.
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form. -
Cellular localization
Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab276741 has not yet been referenced specifically in any publications.