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    recombinant-mouse-cd200r-protein-his-tag-ab219227.pdf

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Immunology Adaptive Immunity T Cells CD
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Recombinant mouse CD200R protein (His tag) (ab219227)

  • Datasheet
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SDS-PAGE - Recombinant Mouse CD200R protein (His tag) (ab219227)
  • Functional Studies - Recombinant Mouse CD200R protein (His tag) (ab219227)

Key features and details

  • Expression system: Baculovirus infected insect cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant mouse CD200R protein (His tag)
    See all CD200R proteins and peptides
  • Biological activity

    Mouse CD200 coated at 1 µg/mL (100 µL/well) can bind ab219227. The ED50 range ≤ 1 µg/mL.

  • Purity

    > 95 % SDS-PAGE.
    ab219227 was purified by using conventional chromatography techniques.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Baculovirus infected insect cells
  • Accession

    Q9ES57
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      ADPTDKNQTT QNNSSSPLTQ VNTTVSVQIG TKALLCCFSI PLTKAVLITW IIKLRGLPSC TIAYKVDTKT NETSCLGRNI TWASTPDHSP ELQISAVTLQ HEGTYTCETV TPEGNFEKNY DLQVLVPPEV TYFPEKNRSA VCEAMAGKPA AQISWSPDGD CVTTSESHSN GTVTVRSTCH WEQNNVSDVS CIVSHLTGNQ SLSIELSRGG NQSLRPLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH
    • Predicted molecular weight

      50 kDa including tags
    • Amino acids

      26 to 241
    • Tags

      His tag C-Terminus
    • Additional sequence information

      Tagged hIgG-His.

Associated products

  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-6X His tag® antibody (ab9108)

Specifications

Our Abpromise guarantee covers the use of ab219227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: PBS, 10% Glycerol (glycerin, glycerine)

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CD 200R
    • CD200 cell surface glycoprotein receptor
    • CD200 receptor 1
    • Cd200r1
    • Cell surface glycoprotein CD200 receptor 1
    • Cell surface glycoprotein OX2 receptor
    • Cell surface glycoprotein OX2 receptor 1
    • cell surface glycoprotein receptor CD200
    • CRTR 2
    • CRTR2
    • HCRTR2
    • MO2R1_HUMAN
    • MOX2 receptor
    • MOX2R
    • OX2R
    see all
  • Function

    Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
  • Tissue specificity

    Expressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.
  • Sequence similarities

    Belongs to the CD200R family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q8TD46 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant Mouse CD200R protein (His tag) (ab219227)
    SDS-PAGE - Recombinant Mouse CD200R protein (His tag) (ab219227)

    15% SDS-PAGE - Recombinant Mouse CD200R protein (ab219227) at 3 μg.

  • Functional Studies - Recombinant Mouse CD200R protein (His tag) (ab219227)
    Functional Studies - Recombinant Mouse CD200R protein (His tag) (ab219227)

    Mouse CD200 coated at 1 µg/mL (100 µL/well) can bind Mouse CD200 R1 (ab219227). The ED50 range ≤ 1 µg/mL.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab219227? Please let us know so that we can cite the reference in this datasheet.

    ab219227 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab219227.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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