Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
Product nameRecombinant mouse CD272/BTLA protein (Active)
See all CD272/BTLA proteins and peptides
Measured by its binding ability in a functional ELISA. Immobilized ab220466 at 5 μg/ml (100 μl/well) can bind Mouse B7-H4, Fc Tag with a linear range of 0.05-0.8 μg/ml.
Purity> 95 % SDS-PAGE.
Endotoxin level< 1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceEKATKRNDEECEVQLNIKRNSKHSAWTGELFKIECPVKYCVHRPNVTWCK HNGTIWVPLEVGPQLYTSWEENRSVPVFVLHFKPIHLSDNGSYSCSTNFN SQVINSHSVTIHVRERTQNSSEHPLIISDIPDATNASGPSTMEKRPG
Predicted molecular weight19 kDa including tags
Amino acids30 to 176
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab220466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as CD272.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
- B and T lymphocyte associated protein
- B and T lymphocyte attenuator
- B and T lymphocyte associated
FunctionLymphocyte inhibitory receptor which inhibits lymphocytes during immune response.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPhosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab220466 has not yet been referenced specifically in any publications.